The dual aptamer approach : rational design of a high-affinity FAD aptamer
| Type of Publication: | Journal article |
| Publication status: | Published |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-0-311505 |
| Author: | Merkle, Tobias; Holder, Isabelle T.; Hartig, Jörg S. |
| Year of publication: | 2015 |
| Published in: | Organic and Biomolecular Chemistry ; 14 (2015), 2. - pp. 447-450. - ISSN 1477-0520. - eISSN 1477-0539 |
| Pubmed ID: | 26586417 |
| DOI (citable link): | https://dx.doi.org/10.1039/C5OB02026C |
| Summary: |
A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.
|
| Subject (DDC): | 540 Chemistry |
| Link to License: | In Copyright |
| Bibliography of Konstanz: | Yes |
Cite This
Files in this item
![[PDF]](/themes/Kops/images/mimes/pdf.png "PDF file"){kind=small}
MERKLE, Tobias, Isabelle T. HOLDER, Jörg S. HARTIG, 2015. The dual aptamer approach : rational design of a high-affinity FAD aptamer. In: Organic and Biomolecular Chemistry. 14(2), pp. 447-450. ISSN 1477-0520. eISSN 1477-0539. Available under: doi: 10.1039/C5OB02026C
@article{Merkle2015-12-23aptam-32337, title={The dual aptamer approach : rational design of a high-affinity FAD aptamer}, year={2015}, doi={10.1039/C5OB02026C}, number={2}, volume={14}, issn={1477-0520}, journal={Organic and Biomolecular Chemistry}, pages={447--450}, author={Merkle, Tobias and Holder, Isabelle T. and Hartig, Jörg S.} }
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/32337"> <dc:creator>Hartig, Jörg S.</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:rights>terms-of-use</dc:rights> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:issued>2015-12-23</dcterms:issued> <dc:creator>Merkle, Tobias</dc:creator> <dc:contributor>Hartig, Jörg S.</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/32337/1/Merkle_0-311505.pdf"/> <dc:contributor>Merkle, Tobias</dc:contributor> <dc:contributor>Holder, Isabelle T.</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/32337/1/Merkle_0-311505.pdf"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-12-04T09:35:18Z</dc:date> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/29"/> <dcterms:title>The dual aptamer approach : rational design of a high-affinity FAD aptamer</dcterms:title> <dc:creator>Holder, Isabelle T.</dc:creator> <dcterms:abstract xml:lang="eng">A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.</dcterms:abstract> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-12-04T09:35:18Z</dcterms:available> <dc:language>eng</dc:language> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/32337"/> </rdf:Description> </rdf:RDF>
Downloads since Dec 4, 2015 (Information about access statistics)
| Merkle_0-311505.pdf | 281 |
This item appears in the following Collection(s)
Search KOPS
Browse
My Account
