The dual aptamer approach : rational design of a high-affinity FAD aptamer
| Type of Publication: | Journal article |
| Publication status: | Published |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-0-311505 |
| Author: | Merkle, Tobias; Holder, Isabelle T.; Hartig, Jörg S. |
| Year of publication: | 2015 |
| Published in: | Organic and Biomolecular Chemistry ; 14 (2015), 2. - pp. 447-450. - ISSN 1477-0520. - eISSN 1477-0539 |
| Pubmed ID: | 26586417 |
| DOI (citable link): | https://dx.doi.org/10.1039/C5OB02026C |
| Summary: |
A design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.
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| Subject (DDC): | 540 Chemistry |
| Link to License: | Terms of use |
| Bibliography of Konstanz: | Yes |
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MERKLE, Tobias, Isabelle T. HOLDER, Jörg S. HARTIG, 2015. The dual aptamer approach : rational design of a high-affinity FAD aptamer. In: Organic and Biomolecular Chemistry. 14(2), pp. 447-450. ISSN 1477-0520. eISSN 1477-0539. Available under: doi: 10.1039/C5OB02026C
@article{Merkle2015-12-23aptam-32337, title={The dual aptamer approach : rational design of a high-affinity FAD aptamer}, year={2015}, doi={10.1039/C5OB02026C}, number={2}, volume={14}, issn={1477-0520}, journal={Organic and Biomolecular Chemistry}, pages={447--450}, author={Merkle, Tobias and Holder, Isabelle T. and Hartig, Jörg S.} }
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