Type of Publication: | Journal article |
Publication status: | Published |
Author: | Müller, Dieter G.; Gachon, Claire M. M.; Küpper, Frithjof C. |
Year of publication: | 2008 |
Published in: | Cahiers de Biologie Marine ; 49 (2008), 1. - pp. 59-65. - ISSN 2262-3094 |
URL of original publication: | http://application.sb-roscoff.fr/cbm/article.htm?execution=e14s1, Last access on Nov 27, 2015 |
Summary: |
The increasing number of molecular and biotechnological studies involving brown algae has led to a growing demand for bacteria-free cultures. We describe here simple methods to establish axenic clonal cultures of filamentous marine brown algae. Thallus fragments are exposed on agar plates to commercially available paper disks loaded with defined concentrations of antibiotics. Regenerating fragments are isolated and used to initiate axenic algal cultures in liquid medium or on agar. We found the following agents to be tolerated by algae and active against marine bacteria: ciprofloxacin, chloramphenicol, rifampicin, polymyxin B, and kanamycin. Axenic agar cultures are maintained by serial transfer on agar, or re-introduced into liquid medium. Although we recommend transfer of agar cultures in 2-3 month intervals, we have observed survival times on agar of up to 2 years without attention.
|
Subject (DDC): | 570 Biosciences, Biology |
Bibliography of Konstanz: | Yes |
Files | Size | Format | View |
---|---|---|---|
There are no files associated with this item. |
MÜLLER, Dieter G., Claire M. M. GACHON, Frithjof C. KÜPPER, 2008. Axenic clonal cultures of filamentous brown algae : initiation and maintenance. In: Cahiers de Biologie Marine. 49(1), pp. 59-65. ISSN 2262-3094
@article{Muller2008Axeni-32277, title={Axenic clonal cultures of filamentous brown algae : initiation and maintenance}, url={http://application.sb-roscoff.fr/cbm/article.htm?execution=e14s1}, year={2008}, number={1}, volume={49}, issn={2262-3094}, journal={Cahiers de Biologie Marine}, pages={59--65}, author={Müller, Dieter G. and Gachon, Claire M. M. and Küpper, Frithjof C.} }
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/32277"> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/32277"/> <dc:contributor>Müller, Dieter G.</dc:contributor> <dcterms:abstract xml:lang="eng">The increasing number of molecular and biotechnological studies involving brown algae has led to a growing demand for bacteria-free cultures. We describe here simple methods to establish axenic clonal cultures of filamentous marine brown algae. Thallus fragments are exposed on agar plates to commercially available paper disks loaded with defined concentrations of antibiotics. Regenerating fragments are isolated and used to initiate axenic algal cultures in liquid medium or on agar. We found the following agents to be tolerated by algae and active against marine bacteria: ciprofloxacin, chloramphenicol, rifampicin, polymyxin B, and kanamycin. Axenic agar cultures are maintained by serial transfer on agar, or re-introduced into liquid medium. Although we recommend transfer of agar cultures in 2-3 month intervals, we have observed survival times on agar of up to 2 years without attention.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-11-27T10:29:37Z</dc:date> <dc:language>eng</dc:language> <dc:creator>Müller, Dieter G.</dc:creator> <dc:contributor>Küpper, Frithjof C.</dc:contributor> <dc:creator>Küpper, Frithjof C.</dc:creator> <dc:contributor>Gachon, Claire M. M.</dc:contributor> <dc:creator>Gachon, Claire M. M.</dc:creator> <dcterms:issued>2008</dcterms:issued> <dcterms:title>Axenic clonal cultures of filamentous brown algae : initiation and maintenance</dcterms:title> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2015-11-27T10:29:37Z</dcterms:available> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> </rdf:Description> </rdf:RDF>