Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

Kurzanzeige
dc.contributor.authorHeussner, Alexandra H.
dc.contributor.authorAusländer, Simondeu
dc.contributor.authorDietrich, Daniel R.
dc.date.accessioned2011-03-24T17:35:27Zdeu
dc.date.available2011-03-24T17:35:27Zdeu
dc.date.issued2010deu
dc.description.abstractA monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 μg/L (8 nM), a limit of quantification (LOQ) of 3.7 μg/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Toxins 2 (2010), 6, pp. 1582-1594deu
dc.identifier.doi10.3390/toxins2061582
dc.identifier.pmid22069651
dc.identifier.ppn338469095deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/7569
dc.language.isoengdeu
dc.legacy.dateIssued2011deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectmycotoxindeu
dc.subjectochratoxin Bdeu
dc.subjectmonoclonal antibodydeu
dc.subjectELISAdeu
dc.subjectwestern analysisdeu
dc.subject.ddc570deu
dc.titleDevelopment and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISAeng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Heussner2010Devel-7569,
  year={2010},
  doi={10.3390/toxins2061582},
  title={Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA},
  number={6},
  volume={2},
  journal={Toxins},
  pages={1582--1594},
  author={Heussner, Alexandra H. and Ausländer, Simon and Dietrich, Daniel R.}
}
kops.citation.iso690HEUSSNER, Alexandra H., Simon AUSLÄNDER, Daniel R. DIETRICH, 2010. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA. In: Toxins. 2010, 2(6), pp. 1582-1594. eISSN 2072-6651. Available under: doi: 10.3390/toxins2061582deu
kops.citation.iso690HEUSSNER, Alexandra H., Simon AUSLÄNDER, Daniel R. DIETRICH, 2010. Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA. In: Toxins. 2010, 2(6), pp. 1582-1594. eISSN 2072-6651. Available under: doi: 10.3390/toxins2061582eng
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