Publikation:

A flexible loop in the paxillin LIM3 domain mediates its direct binding to integrin β subunits

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2024

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Deutsche Forschungsgemeinschaft (DFG): CRC969, project B06
Deutsche Forschungsgemeinschaft (DFG): RTG 2473 Project C3
Deutsche Forschungsgemeinschaft (DFG): RTG 2474
Deutsche Forschungsgemeinschaft (DFG): RTG 2474

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PLoS Biology. Public Library of Science (PLoS). 2024, 22(9), e3002757. ISSN 1544-9173. eISSN 1545-7885. Verfügbar unter: doi: 10.1371/journal.pbio.3002757

Zusammenfassung

Integrins are fundamental for cell adhesion and the formation of focal adhesions (FA). Accordingly, these receptors guide embryonic development, tissue maintenance, and haemostasis but are also involved in cancer invasion and metastasis. A detailed understanding of the molecular interactions that drive integrin activation, FA assembly, and downstream signalling cascades is critical. Here, we reveal a direct association of paxillin, a marker protein of FA sites, with the cytoplasmic tails of the integrin β1 and β3 subunits. The binding interface resides in paxillin’s LIM3 domain, where based on the NMR structure and functional analyses, a flexible, 7-amino acid loop engages the unstructured part of the integrin cytoplasmic tail. Genetic manipulation of the involved residues in either paxillin or integrin β3 compromises cell adhesion and motility of murine fibroblasts. This direct interaction between paxillin and the integrin cytoplasmic domain identifies an alternative, kindlin-independent mode of integrin outside-in signalling particularly important for integrin β3 function.

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570 Biowissenschaften, Biologie

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ISO 690BAADE, Timo, Marcus MICHAELIS, Andreas PRESTEL, Christoph PAONE, Nikolai KLISHIN, Marleen HERBINGER, Laura SCHEINOST, Ruslan NEDIELKOV, Christof R. HAUCK, Heiko M. MÖLLER, 2024. A flexible loop in the paxillin LIM3 domain mediates its direct binding to integrin β subunits. In: PLoS Biology. Public Library of Science (PLoS). 2024, 22(9), e3002757. ISSN 1544-9173. eISSN 1545-7885. Verfügbar unter: doi: 10.1371/journal.pbio.3002757
BibTex
@article{Baade2024-09-04flexi-71231,
  title={A flexible loop in the paxillin LIM3 domain mediates its direct binding to integrin β subunits},
  year={2024},
  doi={10.1371/journal.pbio.3002757},
  number={9},
  volume={22},
  issn={1544-9173},
  journal={PLoS Biology},
  author={Baade, Timo and Michaelis, Marcus and Prestel, Andreas and Paone, Christoph and Klishin, Nikolai and Herbinger, Marleen and Scheinost, Laura and Nedielkov, Ruslan and Hauck, Christof R. and Möller, Heiko M.},
  note={Article Number: e3002757}
}
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The ImageJ macro used to quantify the cell spreading data has been deposited to the Zenodo database
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