Structurally different rat liver medium-chain acyl-CoA dehydrogenase directed by complementary DNA's differing in their 5'-region

Kurzanzeige
dc.contributor.authorInagaki, Taisukedeu
dc.contributor.authorOhishi, Nobukodeu
dc.contributor.authorTsukagoshi, Norihirodeu
dc.contributor.authorUdaka, Shigezodeu
dc.contributor.authorGhisla, Sandro
dc.contributor.authorYagi, Kuniodeu
dc.date.accessioned2011-03-24T17:37:03Zdeu
dc.date.available2011-03-24T17:37:03Zdeu
dc.date.issued1991deu
dc.description.abstractDifferent forms of rat liver medium-chain acyl CoA dehydrogenase (MCAD) (EC 1.3.99.3) were produced in Escherichia coli carrying expression plasmids (pRMCADm-1−9) differing at the 5′-region of the cDNA. The proteins expressed could be readily extracted from the cells. The protein (not, vert, similar44 kDa) directed by pRMCADm-3 showed the highest activity and was readily purified to homogeneity. The purified enzyme contained non-covalently bound FAD and was similar to rat liver mitochondrial enzyme in all respects examined. The purified protein (not, vert, similar45 kDa) directed by pRMCADm-1 did not contain FAD and showed no enzymatic activity. Therefore, the leader peptide disturbs the binding of FAD to the apoprotein. The purified protein (not, vert, similar40 kDa) directed by pRMCADm-6 did not contain FAD. Thus, the deletion of the NH2-terminal portion of the apoprotein to some extent results in its inability to combine with FAD.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Biochimica et Biophysica Acta / Protein Structure and Molecular Enzymology, 1077 (1991), 3, pp. 285-290deu
dc.identifier.doi10.1016/0167-4838(91)90542-8
dc.identifier.ppn279385099deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/7735
dc.language.isoengdeu
dc.legacy.dateIssued2008deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subjectMedium-chaindeu
dc.subjectacyl CoA dehydrogenasedeu
dc.subjectComplementary DNAdeu
dc.subjectRat liverdeu
dc.subject.ddc570deu
dc.titleStructurally different rat liver medium-chain acyl-CoA dehydrogenase directed by complementary DNA's differing in their 5'-regioneng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Inagaki1991Struc-7735,
  year={1991},
  doi={10.1016/0167-4838(91)90542-8},
  title={Structurally different rat liver medium-chain acyl-CoA dehydrogenase directed by complementary DNA's differing in their 5'-region},
  number={3},
  volume={1077},
  issn={0167-4838},
  journal={Biochimica et Biophysica Acta / Protein Structure and Molecular Enzymology},
  pages={285--290},
  author={Inagaki, Taisuke and Ohishi, Nobuko and Tsukagoshi, Norihiro and Udaka, Shigezo and Ghisla, Sandro and Yagi, Kunio}
}
kops.citation.iso690INAGAKI, Taisuke, Nobuko OHISHI, Norihiro TSUKAGOSHI, Shigezo UDAKA, Sandro GHISLA, Kunio YAGI, 1991. Structurally different rat liver medium-chain acyl-CoA dehydrogenase directed by complementary DNA's differing in their 5'-region. In: Biochimica et Biophysica Acta / Protein Structure and Molecular Enzymology. 1991, 1077(3), pp. 285-290. ISSN 0167-4838. Available under: doi: 10.1016/0167-4838(91)90542-8deu
kops.citation.iso690INAGAKI, Taisuke, Nobuko OHISHI, Norihiro TSUKAGOSHI, Shigezo UDAKA, Sandro GHISLA, Kunio YAGI, 1991. Structurally different rat liver medium-chain acyl-CoA dehydrogenase directed by complementary DNA's differing in their 5'-region. In: Biochimica et Biophysica Acta / Protein Structure and Molecular Enzymology. 1991, 1077(3), pp. 285-290. ISSN 0167-4838. Available under: doi: 10.1016/0167-4838(91)90542-8eng
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