@article{Miller2002Reduc-7303,
  year={2002},
  doi={10.1016/S0301-472X(02)00831-7},
  title={Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood  T lymphocytes},
  number={7},
  volume={30},
  issn={0301-472X},
  journal={Experimental Hematology},
  pages={738--744},
  author={Miller, Robin E. and Fayen, John D. and Mohammad, Shaden F. and Stein, Kevin R. and Kadereit, Suzanne and Daum-Woods, Kathleen and Sramkoski, R. Michael and Jacobberger, James W. and Templeton, Dennis and Shurin, Susan B. and Laughlin, Mary J.}
}

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    <dc:creator>Stein, Kevin R.</dc:creator>
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    <dc:contributor>Kadereit, Suzanne</dc:contributor>
    <dc:creator>Kadereit, Suzanne</dc:creator>
    <dc:contributor>Miller, Robin E.</dc:contributor>
    <dc:creator>Jacobberger, James W.</dc:creator>
    <dc:contributor>Jacobberger, James W.</dc:contributor>
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    <dcterms:bibliographicCitation>First publ. in: Experimental Hematology 30 (2002), 7, pp. 738-744</dcterms:bibliographicCitation>
    <dc:creator>Fayen, John D.</dc:creator>
    <dc:creator>Mohammad, Shaden F.</dc:creator>
    <dc:contributor>Sramkoski, R. Michael</dc:contributor>
    <dc:creator>Daum-Woods, Kathleen</dc:creator>
    <dc:contributor>Templeton, Dennis</dc:contributor>
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    <dc:contributor>Daum-Woods, Kathleen</dc:contributor>
    <dc:creator>Templeton, Dennis</dc:creator>
    <dcterms:abstract xml:lang="eng">Objective: A favorable incidence and severity of graft-vs-host disease is observed in patients transplanted with banked, unrelated, HLA-mismatched umbilical cord blood (UCB) grafts, while the incidence of malignant relapse remains low. CTLA-4 mediates negative T-cell signaling and may contribute to the development of allogeneic tolerance. In this study, we compared protein and mRNA expression of CTLA-4 in stimulated UCB and adult peripheral blood T cells.&lt;br /&gt;&lt;br /&gt;Materials and Methods: T cells were isolated from UCB and adult peripheral blood and stimulated with anti-CD3 and anti-CD28 monoclonal antibodies. Cells were immunostained and analyzed by flow cytometry for both surface and intracellular expression of CTLA-4 in the presence and absence of cyclosporin A, and kinetics of CTLA-4 expression compared. CTLA-4 mRNA expression was measured using quantitative real-time polymerase chain reaction. NFAT1 protein levels were measured by Western blot analysis.&lt;br /&gt;&lt;br /&gt;Results: These studies demonstrate reduced surface and intracellular expression of CTLA-4 in stimulated UCB T cells compared to adult controls. Furthermore, reduced CTLA-4 protein expression in UCB T cells was noted to be in part transcriptionally regulated, as CTLA-4 mRNA levels also were significantly lower. Reduced CLTA-4 expression by UCB T cells followed the kinetics of delayed and reduced expression of the transcription factor NFAT1 by UCB T lymphocytes during primary stimulation. Moreover, cyclosporin A, which is known to modulate NFAT activation, reduced CTLA-4 protein expression in adult and UCB T cells.&lt;br /&gt;&lt;br /&gt;Conclusion: Reduced expression of the key regulatory proteins CTLA-4 and NFAT-1 may contribute to favorable UCB T lymphocyte allogeneic responses.</dcterms:abstract>
    <dc:contributor>Mohammad, Shaden F.</dc:contributor>
    <dc:contributor>Stein, Kevin R.</dc:contributor>
    <dc:contributor>Shurin, Susan B.</dc:contributor>
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    <dc:contributor>Laughlin, Mary J.</dc:contributor>
    <dcterms:title>Reduced CTLA-4 protein and messenger RNA expression in umbilical cord blood  T lymphocytes</dcterms:title>
    <dc:creator>Sramkoski, R. Michael</dc:creator>
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    <dc:contributor>Fayen, John D.</dc:contributor>
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