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Fast, Sensitive, and Inexpensive Alternative to Analytical Pigment HPLC:Quantification of Chlorophylls and Carotenoids in Crude Extracts by Fitting with Gauss Peak Spectra

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2007

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Parameswaran, Aravind

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-118822
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https://doi.org/10.1021/ac070236m
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Analytical Chemistry. 2007, 79(20), pp. 7611-7627. ISSN 0003-2700. eISSN 1520-6882. Available under: doi: 10.1021/ac070236m

Zusammenfassung

Quantification of pigments in complex mixtures is an important task in the physiology of photosynthetic organisms, because pigment composition differs depending on the species, tissue, and physiological state. Currently available methods, however, are either limited to very few pigments (classical UV/vis spectroscopic methods), or they are time-consuming, labor intensive, or costly (e.g., HPLC). Here we describe a UV/vis spectrophotometric method that is capable of a rapid (1 min/sample) and inexpensive (

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570 Biowissenschaften, Biologie

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ISO 690KÜPPER, Hendrik, Sven SEIBERT, Aravind PARAMESWARAN, 2007. Fast, Sensitive, and Inexpensive Alternative to Analytical Pigment HPLC:Quantification of Chlorophylls and Carotenoids in Crude Extracts by Fitting with Gauss Peak Spectra. In: Analytical Chemistry. 2007, 79(20), pp. 7611-7627. ISSN 0003-2700. eISSN 1520-6882. Available under: doi: 10.1021/ac070236m
BibTex
@article{Kupper2007Sensi-1151,
  year={2007},
  doi={10.1021/ac070236m},
  title={Fast, Sensitive, and Inexpensive Alternative to Analytical Pigment HPLC:Quantification of Chlorophylls and Carotenoids in Crude Extracts by Fitting with Gauss Peak Spectra},
  number={20},
  volume={79},
  issn={0003-2700},
  journal={Analytical Chemistry},
  pages={7611--7627},
  author={Küpper, Hendrik and Seibert, Sven and Parameswaran, Aravind}
}
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