Saccharin as a sole source of carbon and energy for Sphingomonas xenophaga SKN

Schleheck, David; Cook, Alasdair M.

Saccharin as a sole source of carbon and energy for Sphingomonas xenophaga SKN

Files

fulltext2.pdf(224.45 KB)

Date

2003

Publication type

Journal article

Published in

Archives of Microbiology ; 179 (2003), 3. - pp. 191-196. - ISSN 0302-8933

Abstract

A bacterium, strain SKN, that was able to utilize saccharin as the sole source of carbon and energy for aerobic growth, was enriched and isolated from communal sewage. The isolate was identified as a strain of Sphingomonas xenophaga. Saccharin was quantitatively converted to cell material, sulfate, ammonium and, presumably, CO(2). The specific rate of saccharin-dependent oxygen uptake during growth reached a maximum before the culture entered the stationary phase and then fell to undetectable levels. Saccharin was degraded only in the presence of molecular oxygen. Catechol was detected as an intermediate during degradation of saccharin in whole cells and catechol 1,2-dioxygenase was expressed inducibly during growth with saccharin. There was an apparent requirement of 2 mol O(2)/mol saccharin to remove the substituents on the ring and to cleave the ring. We presume that S. xenophaga SKN synthesizes a multi-component saccharin dioxygenase that simultaneously cleaves off both vicinal substituents from the aromatic ring to yield catechol and the undefined precursor of CO(2) as well as sulfate and ammonium ions.

Subject (DDC)

570 Biosciences, Biology

Keywords

Saccharin degradation,Saccharin dioxygenase,Sphingomonas xenophaga,Catechol 1,2 dioxygenase

Cite This

ISO 690

SCHLEHECK, David, Alasdair M. COOK, 2003. Saccharin as a sole source of carbon and energy for Sphingomonas xenophaga SKN. In: Archives of Microbiology. 179(3), pp. 191-196. ISSN 0302-8933. Available under: doi: 10.1007/s00203-002-0515-2

BibTex

@article{Schleheck2003Sacch-8069,
  year={2003},
  doi={10.1007/s00203-002-0515-2},
  title={Saccharin as a sole source of carbon and energy for Sphingomonas xenophaga SKN},
  number={3},
  volume={179},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={191--196},
  author={Schleheck, David and Cook, Alasdair M.}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8069">
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8069/1/fulltext2.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:42Z</dc:date>
    <dcterms:bibliographicCitation>First publ. in:  Archives of Microbiology 179 (2003), 3, pp. 191-196</dcterms:bibliographicCitation>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:39:42Z</dcterms:available>
    <dc:creator>Schleheck, David</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:format>application/pdf</dc:format>
    <dc:contributor>Cook, Alasdair M.</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8069"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Schleheck, David</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8069/1/fulltext2.pdf"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:language>eng</dc:language>
    <dc:creator>Cook, Alasdair M.</dc:creator>
    <dcterms:title>Saccharin as a sole source of carbon and energy for Sphingomonas xenophaga SKN</dcterms:title>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2003</dcterms:issued>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:abstract xml:lang="eng">A bacterium, strain SKN, that was able to utilize saccharin as the sole source of carbon and energy for aerobic growth, was enriched and isolated from communal sewage. The isolate was identified as a strain of Sphingomonas xenophaga. Saccharin was quantitatively converted to cell material, sulfate, ammonium and, presumably, CO(2). The specific rate of saccharin-dependent oxygen uptake during growth reached a maximum before the culture entered the stationary phase and then fell to undetectable levels. Saccharin was degraded only in the presence of molecular oxygen. Catechol was detected as an intermediate during degradation of saccharin in whole cells and catechol 1,2-dioxygenase was expressed inducibly during growth with saccharin. There was an apparent requirement of 2 mol O(2)/mol saccharin to remove the substituents on the ring and to cleave the ring. We presume that S. xenophaga SKN synthesizes a multi-component saccharin dioxygenase that simultaneously cleaves off both vicinal substituents from the aromatic ring to yield catechol and the undefined precursor of CO(2) as well as sulfate and ammonium ions.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Bibliography of Konstanz

Yes