@article{Miranda2004Dynam-8343,
  year={2004},
  title={Dynamics of polymorphism of acidocalcisomes in Leishmania parasites},
  number={5},
  volume={121},
  issn={0948-6143},
  journal={Histochemistry and Cell Biology},
  pages={407--418},
  author={Miranda, Kildare and Docampo, Roberto and Grillo, Orlando and Franzen, Anderson and Attias, Márcia and Vercesi, Anibal and Plattner, Helmut and Hentschel, Joachim and Souza, Wanderley de}
}

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    <dc:creator>Miranda, Kildare</dc:creator>
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    <dc:creator>Attias, Márcia</dc:creator>
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    <dc:creator>Plattner, Helmut</dc:creator>
    <dc:contributor>Hentschel, Joachim</dc:contributor>
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    <dcterms:title>Dynamics of polymorphism of acidocalcisomes in Leishmania parasites</dcterms:title>
    <dc:contributor>Plattner, Helmut</dc:contributor>
    <dc:contributor>Franzen, Anderson</dc:contributor>
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    <dcterms:abstract xml:lang="eng">Growth of Leishmania mexicana amazonensis promastigotes in different culture media resulted in structurally and chemically different acidocalcisomes. When grown in SDM-79 medium, the promastigotes showed large spherical acidocalcisomes of up to 1.2 mm diameter distributed throughout the cell. X ray microanalysis and elemental mapping of the organelles showed large amounts of oxygen, phosphorus, sodium, potassium, magnesium, calcium, and zinc. Immunofluorescence microscopy using antisera raised against a peptide sequence of the vacuolar-type proton pyrophosphatase of Arabidopsis thaliana that is conserved in the Leishmania enzyme, indicated localization in acidocalcisomes. When cells were transferred to Warren s medium, the acidocalcisomes transformed from spherical into branched tubular organelles. The labeling pattern of the vacuolar proton-pyrophosphatase, considered as a marker for the organelle, changed accompanying the structural changes of the acidocalcisomes, and the enzyme showed an apparently lower proton-transporting activity when measured in digitonin-permeabilized promastigotes. X-ray microanalysis and elemental mapping of these structures revealed the additional presence of iron. Together, the results reveal that the morphology and composition of acidocalcisomes are greatly influenced by the culture con-ditions.</dcterms:abstract>
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    <dcterms:bibliographicCitation>First publ. in: Histochemistry and Cell Biology 212 (2004), pp. 407-418</dcterms:bibliographicCitation>
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