@article{Koster1995Human-7375,
  year={1995},
  title={Human pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor-1 alpha},
  number={2},
  volume={231},
  issn={0014-2956},
  journal={European Journal of Biochemistry},
  pages={414--423},
  author={Köster, Sandra and Thöny, Beat and Macheroux, Peter and Curtius, Hans-Christoph and Heizmann, Claus W. and Pfleiderer, Wolfgang and Ghisla, Sandro}
}

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7375">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:55Z</dcterms:available>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Pfleiderer, Wolfgang</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Thöny, Beat</dc:creator>
    <dc:creator>Macheroux, Peter</dc:creator>
    <dc:contributor>Köster, Sandra</dc:contributor>
    <dc:contributor>Curtius, Hans-Christoph</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:bibliographicCitation>First publ. in: European Journal of Biochemistry 231 (1995), 2, pp. 414-423</dcterms:bibliographicCitation>
    <dc:contributor>Pfleiderer, Wolfgang</dc:contributor>
    <dc:creator>Curtius, Hans-Christoph</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:creator>Heizmann, Claus W.</dc:creator>
    <dcterms:abstract xml:lang="eng">Pterin-4a-carbinolamine dehydratase/dimerization cofactor for hepatocyte nuclear factor-1α is a protein with two different functions. We have overexpressed and purified the human wild-type protein, and its Cys81Ser and Cys81Arg mutants. The Cys81Arg mutant has been proposed to be causative in a hyperphenylalaninaemic patient [Citron, B. A., Kaufman, S., Milstien, S., Naylor, E. W., Greene, C. L. &amp; Davis, M. D. (1993) Am. J. Hum. Genet. 53, 768 774]. The dehydratase behaves as a tetramer on gel filtration, while cross-linking experiments showed mono-, di-, tri-, and tetrameric forms, irrespective of the presence of the single Cys81. Sulfhydryl-modifying reagents did not affect the activity, but rather showed that Cys81 is exposed. Various pterins bind and quench the tryptophan fluorescence suggesting the presence of a specific binding site. The fluorescence is destroyed upon light irradiation. Wild-type and the Cys81Ser protein enhance the rate of the phenylalanine hydroxylase assay ≈ 10-fold, a value similar to that of native dehydratase from rat liver; the Cys81Arg mutant, in contrast, has significantly lower activity. This is compatible with the hypothesis that the dehydratase is a rate-limiting factor for the in vivo phenylalanine hydroxylase reaction. The three proteins enhance the spontaneous dehydration of the synthetic substrate 6,6-dimethyl-7,8-dihydropterin-4a-carbinolamine ≈50 70-fold at 4°C and pH 8.5. The results are discussed in view of the recently solved three-dimensional structure of the enzyme [Ficner, R., Sauer, U. W., Stier, G. &amp; Suck, D. (1995) EMBO J. 14, 2032 2042].</dcterms:abstract>
    <dc:contributor>Heizmann, Claus W.</dc:contributor>
    <dc:contributor>Macheroux, Peter</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dc:language>eng</dc:language>
    <dcterms:title>Human pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor-1 alpha</dcterms:title>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7375/1/Human_Pterin_4_alpha_Carbinolamine_Dehydratase.pdf"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7375"/>
    <dc:format>application/pdf</dc:format>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:55Z</dc:date>
    <dcterms:issued>1995</dcterms:issued>
    <dc:contributor>Thöny, Beat</dc:contributor>
    <dc:creator>Ghisla, Sandro</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7375/1/Human_Pterin_4_alpha_Carbinolamine_Dehydratase.pdf"/>
    <dc:creator>Köster, Sandra</dc:creator>
  </rdf:Description>
</rdf:RDF>