SOFISM : Super-resolution optical fluctuation image scanning microscopy

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2020
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Sroda, Aleksandra
Makowski, Adrian
Rossman, Uri
Lubin, Gur
Oron, Dan
Lapkiewicz, Radek
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Optica. Optica Publishing Group. 2020, 7(10), pp. 1308-1316. eISSN 2334-2536. Available under: doi: 10.1364/OPTICA.399600
Zusammenfassung

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy methods have become a common tool in life science imaging, new methods, supported by cutting-edge technology, continue to emerge. One rather recent addition to the super-resolution toolbox, image scanning microscopy (ISM), achieves an up to twofold lateral resolution enhancement in a robust and straightforward manner. To further enhance ISM's resolution in all three dimensions, we present and experimentally demonstrate here super-resolution optical fluctuation image scanning microscopy (SOFISM). Measuring the fluorescence fluctuation contrast in an ISM architecture, we obtain images with a x2.5 lateral resolution beyond the diffraction limit along with an enhanced axial resolution for a fixed cell sample labeled with commercially available quantum dots. The inherent temporal averaging of the ISM technique enables image acquisition of the fluctuation correlation contrast within millisecond scale pixel dwell times. SOFISM can therefore offer a robust path to achieve high resolution images within a slightly modified confocal microscope, using standard fluorescent labels and within reasonable acquisition times.

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ISO 690SRODA, Aleksandra, Adrian MAKOWSKI, Ron TENNE, Uri ROSSMAN, Gur LUBIN, Dan ORON, Radek LAPKIEWICZ, 2020. SOFISM : Super-resolution optical fluctuation image scanning microscopy. In: Optica. Optica Publishing Group. 2020, 7(10), pp. 1308-1316. eISSN 2334-2536. Available under: doi: 10.1364/OPTICA.399600
BibTex
@article{Sroda2020-02-01T10:07:55ZSOFIS-57997,
  year={2020},
  doi={10.1364/OPTICA.399600},
  title={SOFISM : Super-resolution optical fluctuation image scanning microscopy},
  number={10},
  volume={7},
  journal={Optica},
  pages={1308--1316},
  author={Sroda, Aleksandra and Makowski, Adrian and Tenne, Ron and Rossman, Uri and Lubin, Gur and Oron, Dan and Lapkiewicz, Radek}
}
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