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Serial Synchrotron X-Ray Crystallography (SSX)

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2017

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Wang, Meitian

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https://doi.org/10.1007/978-1-4939-7000-1_10
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WLODAWER, Alexander, ed., Zbigniew DAUTER, ed., Mariusz JASKOLSKI, ed.. Protein Crystallography : Methods and Protocols. New York, NY: Humana Press, 2017, pp. 239-272. Methods in Molecular Biology. 1607. ISBN 978-1-4939-6998-2. Available under: doi: 10.1007/978-1-4939-7000-1_10

Zusammenfassung

Prompted by methodological advances in measurements with X-ray free electron lasers, it was realized in the last two years that traditional (or conventional) methods for data collection from crystals of macromolecular specimens can be complemented by synchrotron measurements on microcrystals that would individually not suffice for a complete data set. Measuring, processing, and merging many partial data sets of this kind requires new techniques which have since been implemented at several third-generation synchrotron facilities, and are described here. Among these, we particularly focus on the possibility of in situ measurements combined with in meso crystal preparations and data analysis with the XDS package and auxiliary programs.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Serial synchrotron crystallography (SSX), Microcrystal, Lipidic cubic phase (LCP), In meso in situ, Room temperature (RT), Cryogenic temperature, Data collection, Data quality, Merging, XDS, XSCALE

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ISO 690DIEDERICHS, Kay, Meitian WANG, 2017. Serial Synchrotron X-Ray Crystallography (SSX). In: WLODAWER, Alexander, ed., Zbigniew DAUTER, ed., Mariusz JASKOLSKI, ed.. Protein Crystallography : Methods and Protocols. New York, NY: Humana Press, 2017, pp. 239-272. Methods in Molecular Biology. 1607. ISBN 978-1-4939-6998-2. Available under: doi: 10.1007/978-1-4939-7000-1_10
BibTex
@incollection{Diederichs2017-06-02Seria-39798,
  year={2017},
  doi={10.1007/978-1-4939-7000-1_10},
  title={Serial Synchrotron X-Ray Crystallography (SSX)},
  number={1607},
  isbn={978-1-4939-6998-2},
  publisher={Humana Press},
  address={New York, NY},
  series={Methods in Molecular Biology},
  booktitle={Protein Crystallography : Methods and Protocols},
  pages={239--272},
  editor={Wlodawer, Alexander and Dauter, Zbigniew and Jaskolski, Mariusz},
  author={Diederichs, Kay and Wang, Meitian}
}
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