Mathematical modelling of the automated FADU assay for the quantification of DNA strand breaks and their repair in human peripheral mononuclear blood cells

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2014
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Zusammenfassung

Background
Cells continuously undergo DNA damage from exogenous agents like irradiation or genotoxic chemicals or from endogenous radicals produced by normal cellular metabolic activities. DNA strand breaks are one of the most common genotoxic lesions and they can also arise as intermediates of DNA repair activity. Unrepaired DNA damage can lead to genomic instability, which can massively compromise the health status of organisms. Therefore it is important to measure and quantify DNA damage and its repair.

Results
We have previously published an automated method for measuring DNA strand breaks based on fluorimetric detection of alkaline DNA unwinding [1], and here we present a mathematical model of the FADU assay, which enables to an analytic expression for the relation between measured fluorescence and the number of strand breaks.

Conclusions
Assessment of the formation and also the repair of DNA strand breaks is a crucial functional parameter to investigate genotoxicity in living cells. A reliable and convenient method to quantify DNA strand breakage is therefore of significant importance for a wide variety of scientific fields, e.g. toxicology, pharmacology, epidemiology and medical sciences.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
510 Mathematik
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FADU, DNA repair, DNA strand breaks, Mathematical model
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ISO 690JUNK, Michael, Judy FIERES, Thilo SINDLINGER, Alexander BÜRKLE, Maria MORENO-VILLANUEVA, 2014. Mathematical modelling of the automated FADU assay for the quantification of DNA strand breaks and their repair in human peripheral mononuclear blood cells. In: BMC Biophysics. 2014, 7, 9. eISSN 2046-1682. Available under: doi: 10.1186/s13628-014-0009-z
BibTex
@article{Junk2014Mathe-29061,
  year={2014},
  doi={10.1186/s13628-014-0009-z},
  title={Mathematical modelling of the automated FADU assay for the quantification of DNA strand breaks and their repair in human peripheral mononuclear blood cells},
  volume={7},
  journal={BMC Biophysics},
  author={Junk, Michael and Fieres, Judy and Sindlinger, Thilo and Bürkle, Alexander and Moreno-Villanueva, Maria},
  note={Article Number: 9}
}
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