Stability of the Thermus thermophilus outer membrane protein TtoA against heat and denaturants

Schmid, Katharina; Odermatt, Meike; Welte, Wolfram; Hauser, Karin

Stability of the Thermus thermophilus outer membrane protein TtoA against heat and denaturants

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2014

Projekt

SFB 969 TP A02: Untersuchung der Strukturbildung von Proteinen mittels zeitaufgelöster Infrarot-Spektroskopie

Sammlungen

Chemie
Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Biomedical Spectroscopy and Imaging. 2014, 3(1), pp. 51-56. ISSN 2212-8794. eISSN 2212-8808. Available under: doi: 10.3233/BSI-140063

Zusammenfassung

TtoA is a major outer membrane protein (Omp) with β-barrel structure from the thermophilic eubacterium T. thermophilus. FT-IR spectroscopy and SDS PAGE analysis were used to monitor the stability of detergent-solubilised TtoA under denaturing conditions. Heat as well as the common denaturants urea and SDS were applied to affect the TtoA structure. The protein has proven to be extremely thermostable in its native form. Denaturants likewise only have little effects on the protein's structure. In detail, a SDS concentration of 1% as is typical for Laemmli buffer does not have an impact on the structure of TtoA, even in combination with a temperature of 99°C. The same holds true for urea concentrations below 8 M. Only the combination of highly concentrated urea or SDS in combination with incubating the protein at 99°C for 10 minutes leads to a change of the secondary structure in TtoA.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

SCHMID, Katharina, Meike ODERMATT, Wolfram WELTE, Karin HAUSER, 2014. Stability of the Thermus thermophilus outer membrane protein TtoA against heat and denaturants. In: Biomedical Spectroscopy and Imaging. 2014, 3(1), pp. 51-56. ISSN 2212-8794. eISSN 2212-8808. Available under: doi: 10.3233/BSI-140063

BibTex

@article{Schmid2014Stabi-26805,
  year={2014},
  doi={10.3233/BSI-140063},
  title={Stability of the Thermus thermophilus outer membrane protein TtoA against heat and denaturants},
  number={1},
  volume={3},
  issn={2212-8794},
  journal={Biomedical Spectroscopy and Imaging},
  pages={51--56},
  author={Schmid, Katharina and Odermatt, Meike and Welte, Wolfram and Hauser, Karin}
}

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Universitätsbibliographie

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