Direct Monitoring of β-Sheet Formation in the Outer Membrane Protein TtoA Assisted by TtOmp85
| dc.contributor.author | Schmid, Katharina | |
| dc.contributor.author | Welte, Wolfram | |
| dc.contributor.author | Hauser, Karin | |
| dc.date.accessioned | 2017-02-16T09:58:09Z | |
| dc.date.available | 2017-02-16T09:58:09Z | |
| dc.date.issued | 2016-08-09 | eng |
| dc.description.abstract | Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy was applied to investigate the folding of an outer membrane protein, TtoA, assisted by TtOmp85, both from the thermophilic eubacterium Thermus thermophilus. To directly monitor the formation of β-sheet structure in TtoA and to analyze the function of TtOmp85, we immobilized unfolded TtoA on an ATR crystal. Interaction with TtOmp85 initiated TtoA folding as shown by time-dependent spectra recorded during the folding process. Our ATR-FTIR experiments prove that TtOmp85 possesses specific functionality to assist β-sheet formation of TtoA. We demonstrate the potential of this spectroscopic approach to study the interaction of outer membrane proteins in vitro and in a time-resolved manner. | eng |
| dc.description.version | published | eng |
| dc.identifier.doi | 10.1021/acs.biochem.6b00691 | eng |
| dc.identifier.pmid | 27400268 | eng |
| dc.identifier.uri | https://kops.uni-konstanz.de/handle/123456789/37497 | |
| dc.language.iso | eng | eng |
| dc.subject.ddc | 540 | eng |
| dc.title | Direct Monitoring of β-Sheet Formation in the Outer Membrane Protein TtoA Assisted by TtOmp85 | eng |
| dc.type | JOURNAL_ARTICLE | eng |
| dspace.entity.type | Publication | |
| kops.citation.bibtex | @article{Schmid2016-08-09Direc-37497,
year={2016},
doi={10.1021/acs.biochem.6b00691},
title={Direct Monitoring of β-Sheet Formation in the Outer Membrane Protein TtoA Assisted by TtOmp85},
number={31},
volume={55},
issn={0006-2960},
journal={Biochemistry},
pages={4333--4343},
author={Schmid, Katharina and Welte, Wolfram and Hauser, Karin}
} | |
| kops.citation.iso690 | SCHMID, Katharina, Wolfram WELTE, Karin HAUSER, 2016. Direct Monitoring of β-Sheet Formation in the Outer Membrane Protein TtoA Assisted by TtOmp85. In: Biochemistry. 2016, 55(31), pp. 4333-4343. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/acs.biochem.6b00691 | deu |
| kops.citation.iso690 | SCHMID, Katharina, Wolfram WELTE, Karin HAUSER, 2016. Direct Monitoring of β-Sheet Formation in the Outer Membrane Protein TtoA Assisted by TtOmp85. In: Biochemistry. 2016, 55(31), pp. 4333-4343. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/acs.biochem.6b00691 | eng |
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