Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes

Kurzanzeige
dc.contributor.authorHolzberger, Bastian
dc.contributor.authorStrohmeier, Juliandeu
dc.contributor.authorSiegmund, Vanessa
dc.contributor.authorDiederichsen, Ulfdeu
dc.contributor.authorMarx, Andreas
dc.date.accessioned2012-05-10T09:43:11Zdeu
dc.date.available2012-05-10T09:43:11Zdeu
dc.date.issued2012-05-01
dc.description.abstractFluorescent analogs of the natural nucleobases are widely used as molecular probes for investigating DNA hybridization and topology. In this study the guanosine analogs 8-vinyl- and 8-styryl-2′-deoxyguanosine were synthesized and converted into the corresponding 5′-triphosphates. These C8 modified nucleotides were processed by various DNA polymerases to create fluorescent DNA. Whereas the 8-styryl modified nucleotide somewhat hampers DNA synthesis 8-vinyl-2′-deoxyguanosine is processed by DNA polymerases emphasizing the broad applicability as a molecular probe for fluorescence spectroscopy.eng
dc.description.versionpublished
dc.identifier.citationFirst publ. in: Bioorganic & Medicinal Chemistry Letters ; 22 (2012), 9. - pp. 3136-3139deu
dc.identifier.doi10.1016/j.bmcl.2012.03.056deu
dc.identifier.pmid22483394
dc.identifier.ppn365776319deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/19279
dc.language.isoengdeu
dc.legacy.dateIssued2012-05-10deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subjectDNAdeu
dc.subjectModified nucleotidedeu
dc.subjectDNA polymerasedeu
dc.subjectFluorescent analogdeu
dc.subjectFluorescence spectroscopydeu
dc.subject.ddc540deu
dc.titleEnzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probeseng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Holzberger2012-05-01Enzym-19279,
  year={2012},
  doi={10.1016/j.bmcl.2012.03.056},
  title={Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes},
  number={9},
  volume={22},
  issn={0960-894X},
  journal={Bioorganic & Medicinal Chemistry Letters},
  pages={3136--3139},
  author={Holzberger, Bastian and Strohmeier, Julian and Siegmund, Vanessa and Diederichsen, Ulf and Marx, Andreas}
}
kops.citation.iso690HOLZBERGER, Bastian, Julian STROHMEIER, Vanessa SIEGMUND, Ulf DIEDERICHSEN, Andreas MARX, 2012. Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes. In: Bioorganic & Medicinal Chemistry Letters. 2012, 22(9), pp. 3136-3139. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2012.03.056deu
kops.citation.iso690HOLZBERGER, Bastian, Julian STROHMEIER, Vanessa SIEGMUND, Ulf DIEDERICHSEN, Andreas MARX, 2012. Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes. In: Bioorganic & Medicinal Chemistry Letters. 2012, 22(9), pp. 3136-3139. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2012.03.056eng
kops.citation.rdf
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/19279">
    <dcterms:title>Enzymatic synthesis of 8-vinyl- and 8-styryl-2′-deoxyguanosine modified DNA—novel fluorescent molecular probes</dcterms:title>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Siegmund, Vanessa</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-05-10T09:43:11Z</dcterms:available>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/19279/1/Holzberger_Enzymatic.pdf"/>
    <dc:contributor>Siegmund, Vanessa</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-05-10T09:43:11Z</dc:date>
    <dc:contributor>Diederichsen, Ulf</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Marx, Andreas</dc:creator>
    <dc:language>eng</dc:language>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/19279"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Holzberger, Bastian</dc:creator>
    <dc:creator>Strohmeier, Julian</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Bioorganic &amp; Medicinal Chemistry Letters ; 22 (2012), 9. - pp. 3136-3139</dcterms:bibliographicCitation>
    <dc:contributor>Holzberger, Bastian</dc:contributor>
    <dcterms:abstract xml:lang="eng">Fluorescent analogs of the natural nucleobases are widely used as molecular probes for investigating DNA hybridization and topology. In this study the guanosine analogs 8-vinyl- and 8-styryl-2′-deoxyguanosine were synthesized and converted into the corresponding 5′-triphosphates. These C8 modified nucleotides were processed by various DNA polymerases to create fluorescent DNA. Whereas the 8-styryl modified nucleotide somewhat hampers DNA synthesis 8-vinyl-2′-deoxyguanosine is processed by DNA polymerases emphasizing the broad applicability as a molecular probe for fluorescence spectroscopy.</dcterms:abstract>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:issued>2012-05-01</dcterms:issued>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:contributor>Strohmeier, Julian</dc:contributor>
    <dc:creator>Diederichsen, Ulf</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/19279/1/Holzberger_Enzymatic.pdf"/>
  </rdf:Description>
</rdf:RDF>
kops.description.openAccessopenaccessgreen
kops.flag.knbibliographytrue
kops.identifier.nbnurn:nbn:de:bsz:352-192794deu
kops.sourcefieldBioorganic & Medicinal Chemistry Letters. 2012, <b>22</b>(9), pp. 3136-3139. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2012.03.056deu
kops.sourcefield.plainBioorganic & Medicinal Chemistry Letters. 2012, 22(9), pp. 3136-3139. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2012.03.056deu
kops.sourcefield.plainBioorganic & Medicinal Chemistry Letters. 2012, 22(9), pp. 3136-3139. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2012.03.056eng
kops.submitter.emailkarin.reichardt@uni-konstanz.dedeu
relation.isAuthorOfPublicationc612a19b-d544-44f0-bd38-104228b97c94
relation.isAuthorOfPublication51d1514a-e6df-4ba8-af01-46340966f1cc
relation.isAuthorOfPublication3488d192-4e21-4a69-8956-f7d02d9f9b3a
relation.isAuthorOfPublication.latestForDiscoveryc612a19b-d544-44f0-bd38-104228b97c94
source.bibliographicInfo.fromPage3136
source.bibliographicInfo.issue9
source.bibliographicInfo.toPage3139
source.bibliographicInfo.volume22
source.identifier.eissn1464-3405
source.identifier.issn0960-894X
source.periodicalTitleBioorganic & Medicinal Chemistry Letters

Dateien

Originalbündel

Gerade angezeigt 1 - 1 von 1
Vorschaubild nicht verfügbar
Name:
Holzberger_Enzymatic.pdf
Größe:
2.53 MB
Format:
Adobe Portable Document Format
Holzberger_Enzymatic.pdf
Holzberger_Enzymatic.pdfGröße: 2.53 MBDownloads: 712

Lizenzbündel

Gerade angezeigt 1 - 1 von 1
Vorschaubild nicht verfügbar
Name:
license.txt
Größe:
1.92 KB
Format:
Plain Text
Beschreibung:
license.txt
license.txtGröße: 1.92 KBDownloads: 0

Sammlungen

Chemie: Publikationen