Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides

Kurzanzeige
dc.contributor.authorVerga, Daniela
dc.contributor.authorWelter, Moritz
dc.contributor.authorMarx, Andreas
dc.date.accessioned2016-04-20T08:11:38Z
dc.date.available2016-04-20T08:11:38Z
dc.date.issued2016eng
dc.description.abstractDNA polymerases can efficiently and sequence selectively incorporate oligonucleotide (ODN)-modified nucleotides and the incorporated oligonucleotide strand can be employed as primer in rolling circle amplification (RCA). The effective amplification of the DNA primer by Φ29 DNA polymerase allows the sequence-selective hybridisation of the amplified strand with a G-quadruplex DNA sequence that has horse radish peroxidase-like activity. Based on these findings we develop a system that allows DNA detection with single-base resolution by naked eye.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1016/j.bmcl.2015.12.082eng
dc.identifier.ppn469145978
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/33632
dc.language.isoengeng
dc.rightsterms-of-use
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/
dc.subjectDNA polymerase, Oligonucleotide-modified nucleotide, Rolling circle amplification, Single-base resolution, Naked-eye detectioneng
dc.subject.ddc540eng
dc.titleSequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotideseng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Verga2016Seque-33632,
  year={2016},
  doi={10.1016/j.bmcl.2015.12.082},
  title={Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides},
  number={3},
  volume={26},
  issn={0960-894X},
  journal={Bioorganic & Medicinal Chemistry Letters},
  pages={841--844},
  author={Verga, Daniela and Welter, Moritz and Marx, Andreas}
}
kops.citation.iso690VERGA, Daniela, Moritz WELTER, Andreas MARX, 2016. Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides. In: Bioorganic & Medicinal Chemistry Letters. 2016, 26(3), pp. 841-844. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2015.12.082deu
kops.citation.iso690VERGA, Daniela, Moritz WELTER, Andreas MARX, 2016. Sequence selective naked-eye detection of DNA harnessing extension of oligonucleotide-modified nucleotides. In: Bioorganic & Medicinal Chemistry Letters. 2016, 26(3), pp. 841-844. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2015.12.082eng
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kops.sourcefieldBioorganic & Medicinal Chemistry Letters. 2016, <b>26</b>(3), pp. 841-844. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2015.12.082deu
kops.sourcefield.plainBioorganic & Medicinal Chemistry Letters. 2016, 26(3), pp. 841-844. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2015.12.082deu
kops.sourcefield.plainBioorganic & Medicinal Chemistry Letters. 2016, 26(3), pp. 841-844. ISSN 0960-894X. eISSN 1464-3405. Available under: doi: 10.1016/j.bmcl.2015.12.082eng
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