2-APB and capsazepine-induced Ca2+ influx stimulates clathrin-dependent endocytosis in alveolar epithelial cells
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Calcium as a second messenger influences many cellular and physiological processes. In lung, alveolar type II (ATII) cells sense mechanical stress and respond by Ca2+ dependent release of surfactant, which is essential for respiratory function. Nevertheless, Ca2+ signaling mechanisms in these cells - in particular Ca2+ entry pathways are still poorly understood. Herein, we investigated pharmacological properties of non-voltage-gated Ca2+ channel modulators in ATII and NCI-H441 cells and demonstrate that 2-Aminoethoxydiphenyl-borinate (2-APB) and capsazepine (CPZ) activate Ca2+ entry with pharmacologically distinguishable components. Surprisingly, 2-APB and CPZ activated clathrin dependent endocytosis in ATII and NCI-H441 cells, which was dependent on Ca2+ entry. The internalized material accumulated in non-acidic granules distinct from surfactant containing lamellar bodies (LB). LB exocytosis was not observed under these conditions. Our study demonstrates that 2-APB/CPZ induces Ca2+ entry which unlike ATP- or stretch-induced Ca2+ entry in ATII cells does not activate exocytosis but an opposing endocytotic mechanism.
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USMANI, Shariq M., Giorgio FOIS, Susanne ALBRECHT, Sonja von AULOCK, Paul DIETL, Oliver H. WITTEKINDT, 2010. 2-APB and capsazepine-induced Ca2+ influx stimulates clathrin-dependent endocytosis in alveolar epithelial cells. In: Cellular physiology and biochemistry. 2010, 25(1), pp. 91-102. ISSN 1015-8987. eISSN 1421-9778. Available under: doi: 10.1159/000272064BibTex
@article{Usmani2010capsa-6513, year={2010}, doi={10.1159/000272064}, title={2-APB and capsazepine-induced Ca2+ influx stimulates clathrin-dependent endocytosis in alveolar epithelial cells}, number={1}, volume={25}, issn={1015-8987}, journal={Cellular physiology and biochemistry}, pages={91--102}, author={Usmani, Shariq M. and Fois, Giorgio and Albrecht, Susanne and Aulock, Sonja von and Dietl, Paul and Wittekindt, Oliver H.} }
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