Feeding habits of culicine mosquitoes in the Cameroon lowland forests based on stable isotopes and blood meal analyses

Lade...
Vorschaubild
Dateien
Njabo_265122.pdf
Njabo_265122.pdfGröße: 199.25 KBDownloads: 349
Datum
2013
Autor:innen
Njabo, Kevin Yana
Smith, Thomas B.
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Journal of Parasitology and Vector Biology. 2013, 5(1), pp. 6-12. Available under: doi: 10.5897/JPVB12.005
Zusammenfassung

Mosquito blood feeding behavior is a very significant component of pathogen transmission and determinant of disease epidemiology. Yet, knowledge of foraging ecology of mosquitoes often depends on the presence of undigested blood in the mosquito mid gut. Approximately 36 h after feeding, the blood meal is sufficiently digested to make identification by molecular techniques difficult, leaving a very narrow window in which these methods can be utilized. Here, we investigated the feeding habits of wild caught culicine mosquitoes from four genera, Aedes, Anopheles, Coquillettidia and Mansonia of the lowland rainforests of Cameroon based on the isotopic ratios of nitrogen (δ15N), carbon (δ13C) and sulfur (δ34S). Results showed that unfed mosquitoes had a lower δ13C, δ15N and middle δ34S values than mosquitoes fed with δ13C appearing to be the best element to differentiate between mosquito species that fed on different host species. Isotopic analyses show that the different mosquito genera may be separated based on their diets, suggesting that linking stable isotope-based assays and DNA analysis may be a powerful new tool to investigate mosquito feeding ecology and the dynamics of vector-borne pathogens.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Carbon (δ13C), nitrogen (δ15N), sulfur (δ34S), isotope, feeding habits, mosquitoes, polymerase chain reaction (PCR)
Konferenz
Rezension
undefined / . - undefined, undefined
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Datensätze
Zitieren
ISO 690NJABO, Kevin Yana, Thomas B. SMITH, Elizabeth YOHANNES, 2013. Feeding habits of culicine mosquitoes in the Cameroon lowland forests based on stable isotopes and blood meal analyses. In: Journal of Parasitology and Vector Biology. 2013, 5(1), pp. 6-12. Available under: doi: 10.5897/JPVB12.005
BibTex
@article{Njabo2013Feedi-26512,
  year={2013},
  doi={10.5897/JPVB12.005},
  title={Feeding habits of culicine mosquitoes in the Cameroon lowland forests based on stable isotopes and blood meal analyses},
  number={1},
  volume={5},
  journal={Journal of Parasitology and Vector Biology},
  pages={6--12},
  author={Njabo, Kevin Yana and Smith, Thomas B. and Yohannes, Elizabeth}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/26512">
    <dcterms:bibliographicCitation>Journal of Parasitology and Vector Biology ; 5 (2013), 1. - S. 6–12</dcterms:bibliographicCitation>
    <dcterms:abstract xml:lang="eng">Mosquito blood feeding behavior is a very significant component of pathogen transmission and determinant of disease epidemiology. Yet, knowledge of foraging ecology of mosquitoes often depends on the presence of undigested blood in the mosquito mid gut. Approximately 36 h after feeding, the blood meal is sufficiently digested to make identification by molecular techniques difficult, leaving a very narrow window in which these methods can be utilized. Here, we investigated the feeding habits of wild caught culicine mosquitoes from four genera, Aedes, Anopheles, Coquillettidia and Mansonia of the lowland rainforests of Cameroon based on the isotopic ratios of nitrogen (δ15N), carbon (δ13C) and sulfur (δ34S). Results showed that unfed mosquitoes had a lower δ13C, δ15N and middle δ34S values than mosquitoes fed with δ13C appearing to be the best element to differentiate between mosquito species that fed on different host species. Isotopic analyses show that the different mosquito genera may be separated based on their diets, suggesting that linking stable isotope-based assays and DNA analysis may be a powerful new tool to investigate mosquito feeding ecology and the dynamics of vector-borne pathogens.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-02-24T20:18:38Z</dc:date>
    <dcterms:issued>2013</dcterms:issued>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/26512"/>
    <dc:creator>Smith, Thomas B.</dc:creator>
    <dc:contributor>Yohannes, Elizabeth</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:title>Feeding habits of culicine mosquitoes in the Cameroon lowland forests based on stable isotopes and blood meal analyses</dcterms:title>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/26512/2/Njabo_265122.pdf"/>
    <dc:creator>Yohannes, Elizabeth</dc:creator>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Smith, Thomas B.</dc:contributor>
    <dc:creator>Njabo, Kevin Yana</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-02-24T20:18:38Z</dcterms:available>
    <dc:contributor>Njabo, Kevin Yana</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/26512/2/Njabo_265122.pdf"/>
    <dc:rights>terms-of-use</dc:rights>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Begutachtet
Diese Publikation teilen