A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance

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2020
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Weis, Severin
Kolar, Allison
Sikorski, Johannes
Egert, Markus
Dannenmann, Michael
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Environmental Microbiology. Wiley. 2020, 22(1), pp. 212-228. ISSN 1462-2912. eISSN 1462-2920. Available under: doi: 10.1111/1462-2920.14840
Zusammenfassung

Ammonia released during organic matter mineralization is converted during nitrification to nitrate. We followed spatiotemporal dynamics of the nitrifying microbial community in deep oligotrophic Lake Constance. Depth‐dependent decrease of total ammonium (0.01–0.84 μM) indicated the hypolimnion as the major place of nitrification with 15N‐isotope dilution measurements indicating a threefold daily turnover of hypolimnetic total ammonium. This was mirrored by a strong increase of ammonia‐oxidizing Thaumarchaeota towards the hypolimnion (13%–21% of bacterioplankton) throughout spring to autumn as revealed by amplicon sequencing and quantitative polymerase chain reaction. Ammonia‐oxidizing bacteria were typically two orders of magnitude less abundant and completely ammonia‐oxidizing (comammox) bacteria were not detected. Both, 16S rRNA gene and amoA (encoding ammonia monooxygenase subunit B) analyses identified only one major species‐level operational taxonomic unit (OTU) of Thaumarchaeota (99% of all ammonia oxidizers in the hypolimnion), which was affiliated to Nitrosopumilus spp. The relative abundance distribution of the single Thaumarchaeon strongly correlated to an equally abundant Chloroflexi clade CL500‐11 OTU and a Nitrospira OTU that was one order of magnitude less abundant. The latter dominated among recognized nitrite oxidizers. This extremely low diversity of nitrifiers shows how vulnerable the ecosystem process of nitrification may be in Lake Constance as Central Europe's third largest lake.

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ISO 690HERBER, Janina, Franziska KLOTZ, Benjamin FROMMEYER, Severin WEIS, Dietmar STRAILE, Allison KOLAR, Johannes SIKORSKI, Markus EGERT, Michael DANNENMANN, Michael PESTER, 2020. A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance. In: Environmental Microbiology. Wiley. 2020, 22(1), pp. 212-228. ISSN 1462-2912. eISSN 1462-2920. Available under: doi: 10.1111/1462-2920.14840
BibTex
@article{Herber2020-01singl-47665,
  year={2020},
  doi={10.1111/1462-2920.14840},
  title={A single Thaumarchaeon drives nitrification in deep oligotrophic Lake Constance},
  number={1},
  volume={22},
  issn={1462-2912},
  journal={Environmental Microbiology},
  pages={212--228},
  author={Herber, Janina and Klotz, Franziska and Frommeyer, Benjamin and Weis, Severin and Straile, Dietmar and Kolar, Allison and Sikorski, Johannes and Egert, Markus and Dannenmann, Michael and Pester, Michael}
}
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