Development of twelve novel microsatellite markers in the medicinal mushroom Cordyceps militaris (Ascomycota : Clavicipitaceae) for its strain identification and genetic analysis

Kim, Kee Young; Hong, In Pyo; Kang, Ji Hyoun; Kim, Ki-Hwan; Kim, Iksoo; Lee, Hyuk Je

Development of twelve novel microsatellite markers in the medicinal mushroom Cordyceps militaris (Ascomycota : Clavicipitaceae) for its strain identification and genetic analysis

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2015

Autor:innen

Kim, Kee Young

Hong, In Pyo

Kang, Ji Hyoun

Kim, Ki-Hwan

Kim, Iksoo

Lee, Hyuk Je

DOI (zitierfähiger Link)

https://doi.org/10.1111/1748-5967.12133

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Entomological Research. 2015, 45(6), pp. 328-331. ISSN 1738-2297. eISSN 1748-5967. Available under: doi: 10.1111/1748-5967.12133

Zusammenfassung

Cordyceps militaris (a caterpillar fungus), which belongs to the class Ascomycetes, has extensively been used for medicinal purposes in East Asia. Here, we isolated and characterized 12 microsatellite loci from the medicinal mushroom, C. militaris. Twenty-nine individual samples were taken from a single locality in southwestern Korea and used to characterize the developed markers. The number of alleles of these loci ranged from two to 13 (mean allelic richness = 6.44). Observed heterozygosity and expected heterozygosity in the population ranged from 0.035 to 0.880 and from 0.035 to 0.886, respectively. Five of 12 loci significantly deviated from Hardy–Weinberg Equilibrium, most likely due to heterozygote deficiency caused by inbreeding. Tests of genotypic linkage disequilibrium between the 12 loci showed no significant association of alleles. These microsatellite markers will provide valuable tools for genetic analyses for the strain identification of C. militaris as well as for its resource conservation.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

caterpillar mushroom, Cordyceps militaris, population genetics, tong chug ha cho

Zitieren

ISO 690

KIM, Kee Young, In Pyo HONG, Ji Hyoun KANG, Ki-Hwan KIM, Iksoo KIM, Hyuk Je LEE, 2015. Development of twelve novel microsatellite markers in the medicinal mushroom Cordyceps militaris (Ascomycota : Clavicipitaceae) for its strain identification and genetic analysis. In: Entomological Research. 2015, 45(6), pp. 328-331. ISSN 1738-2297. eISSN 1748-5967. Available under: doi: 10.1111/1748-5967.12133

BibTex

@article{Kim2015Devel-33270,
  year={2015},
  doi={10.1111/1748-5967.12133},
  title={Development of twelve novel microsatellite markers in the medicinal mushroom Cordyceps militaris (Ascomycota : Clavicipitaceae) for its strain identification and genetic analysis},
  number={6},
  volume={45},
  issn={1738-2297},
  journal={Entomological Research},
  pages={328--331},
  author={Kim, Kee Young and Hong, In Pyo and Kang, Ji Hyoun and Kim, Ki-Hwan and Kim, Iksoo and Lee, Hyuk Je}
}

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    <dcterms:abstract xml:lang="eng">Cordyceps militaris (a caterpillar fungus), which belongs to the class Ascomycetes, has extensively been used for medicinal purposes in East Asia. Here, we isolated and characterized 12 microsatellite loci from the medicinal mushroom, C. militaris. Twenty-nine individual samples were taken from a single locality in southwestern Korea and used to characterize the developed markers. The number of alleles of these loci ranged from two to 13 (mean allelic richness = 6.44). Observed heterozygosity and expected heterozygosity in the population ranged from 0.035 to 0.880 and from 0.035 to 0.886, respectively. Five of 12 loci significantly deviated from Hardy–Weinberg Equilibrium, most likely due to heterozygote deficiency caused by inbreeding. Tests of genotypic linkage disequilibrium between the 12 loci showed no significant association of alleles. These microsatellite markers will provide valuable tools for genetic analyses for the strain identification of C. militaris as well as for its resource conservation.</dcterms:abstract>
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