Belowground plant inputs exert higher metabolic activities and carbon use efficiency of soil nematodes than aboveground inputs

Abstract

Soil nematodes are key components of soil food web and, through their metabolic activities, play a crucial role in soil carbon (C) cycling. Aboveground and belowground plant C inputs can directly, or indirectly via soil microbes, modify nematode abundance and community composition. Aboveground and belowground C inputs differ in chemical composition, amounts, and frequency, so we hypothesized that the two input pathways affect nematode communities differently. To assess the relative contributions of aboveground versus belowground inputs to nematode community composition and activity, we subjected grassland soils to four plant input pathways over two consecutive years: no input, only aboveground input (+A), only belowground input (+B), and both aboveground and belowground inputs (+A + B). Nematode metabolic footprints, as estimates of C used in growth/reproduction and C lost by respiration, and C use efficiency (C used/(C used + C lost)) were calculated. We predicted that soils with belowground inputs, which are more directly linked to the soil biota, and which contain a more labile blend of molecules, would support richer and more complex nematode communities, and also favor a bacterial-driven decomposition channel. Accordingly, we showed that + B soils supported higher nematode numbers than + A soils, and that the bacterial decomposition channel was dominant in + B soils, while the fungal decomposition channel dominated in + A soils. Compared with + A soils, +B input system increased nematode structure footprints (the metabolic footprints of nematodes in upper functional guilds) rather than enrichment footprints (the metabolic footprints of enrichment opportunistic nematodes). Moreover, we observed that, compared to + A soils, +B soils had higher growth and respiration rates of bacterivores, omnivores-predators, and total nematodes. Finally, we found higher C use efficiency values for omnivores-predators and total nematodes in + B than in + A soils. We thus conclude that belowground plant-derived resources, by changing the ratio between fungivores and bacterivores, induce a faster carbon turnover rate, and higher metabolic activity of soil nematodes within soil food web, ultimately spurring richer and more efficient soil food web than aboveground inputs.