Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling

dc.contributor.authorGrafahrend-Belau, Eva
dc.contributor.authorJunker, Astrid
dc.contributor.authorSchreiber, Falk
dc.contributor.authorJunker, Björn H.
dc.date.accessioned2017-04-25T07:59:16Z
dc.date.available2017-04-25T07:59:16Z
dc.date.issued2014eng
dc.description.abstractThe analysis of plant metabolic networks essentially contributes to the understanding of the efficiency of plant systems in terms of their biotechnological usage. Metabolic fluxes are determined by biochemical parameters such as metabolite concentrations as well as enzyme properties and activities, which in turn are the result of various regulatory events at various levels between control of transcription and posttranslational regulation of enzyme protein activity. Thus, knowledge about metabolic fluxes on a large scale provides an integrated view on the functional state of a metabolically active cell, organ, or system. In this chapter, we introduce flux balance analysis as a constraint-based method for the prediction of optimal metabolic fluxes in a given metabolic network. Furthermore, we provide a step-by-step protocol for metabolic network reconstruction and constraint-based analysis using the COBRA Toolbox.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1007/978-1-62703-688-7_17eng
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/38590
dc.language.isoengeng
dc.subjectFlux balance analysis, Metabolic network reconstruction, Matlab, COBRA toolboxeng
dc.subject.ddc004eng
dc.titleFlux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labelingeng
dc.typeINCOLLECTIONeng
dspace.entity.typePublication
kops.citation.bibtex
@incollection{GrafahrendBelau2014Balan-38590,
  year={2014},
  doi={10.1007/978-1-62703-688-7_17},
  title={Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling},
  number={1090},
  isbn={978-1-62703-687-0},
  publisher={Humana Press},
  address={Totowa, NJ},
  series={Methods in Molecular Biology},
  booktitle={Plant Metabolic Flux Analysis},
  pages={281--299},
  editor={Dieuaide-Noubhani, Martine and Alonso, Ana Paula},
  author={Grafahrend-Belau, Eva and Junker, Astrid and Schreiber, Falk and Junker, Björn H.}
}
kops.citation.iso690GRAFAHREND-BELAU, Eva, Astrid JUNKER, Falk SCHREIBER, Björn H. JUNKER, 2014. Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling. In: DIEUAIDE-NOUBHANI, Martine, ed., Ana Paula ALONSO, ed.. Plant Metabolic Flux Analysis. Totowa, NJ: Humana Press, 2014, pp. 281-299. Methods in Molecular Biology. 1090. ISBN 978-1-62703-687-0. Available under: doi: 10.1007/978-1-62703-688-7_17deu
kops.citation.iso690GRAFAHREND-BELAU, Eva, Astrid JUNKER, Falk SCHREIBER, Björn H. JUNKER, 2014. Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling. In: DIEUAIDE-NOUBHANI, Martine, ed., Ana Paula ALONSO, ed.. Plant Metabolic Flux Analysis. Totowa, NJ: Humana Press, 2014, pp. 281-299. Methods in Molecular Biology. 1090. ISBN 978-1-62703-687-0. Available under: doi: 10.1007/978-1-62703-688-7_17eng
kops.citation.rdf
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/38590">
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/38590"/>
    <dc:contributor>Junker, Björn H.</dc:contributor>
    <dc:creator>Grafahrend-Belau, Eva</dc:creator>
    <dc:contributor>Schreiber, Falk</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-04-25T07:59:16Z</dcterms:available>
    <dc:creator>Junker, Astrid</dc:creator>
    <dc:creator>Schreiber, Falk</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:issued>2014</dcterms:issued>
    <dc:creator>Junker, Björn H.</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-04-25T07:59:16Z</dc:date>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/36"/>
    <dc:contributor>Grafahrend-Belau, Eva</dc:contributor>
    <dc:contributor>Junker, Astrid</dc:contributor>
    <dcterms:abstract xml:lang="eng">The analysis of plant metabolic networks essentially contributes to the understanding of the efficiency of plant systems in terms of their biotechnological usage. Metabolic fluxes are determined by biochemical parameters such as metabolite concentrations as well as enzyme properties and activities, which in turn are the result of various regulatory events at various levels between control of transcription and posttranslational regulation of enzyme protein activity. Thus, knowledge about metabolic fluxes on a large scale provides an integrated view on the functional state of a metabolically active cell, organ, or system. In this chapter, we introduce flux balance analysis as a constraint-based method for the prediction of optimal metabolic fluxes in a given metabolic network. Furthermore, we provide a step-by-step protocol for metabolic network reconstruction and constraint-based analysis using the COBRA Toolbox.</dcterms:abstract>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/36"/>
    <dcterms:title>Flux Balance Analysis as an Alternative Method to Estimate Fluxes Without Labeling</dcterms:title>
  </rdf:Description>
</rdf:RDF>
kops.flag.knbibliographyfalse
kops.sourcefieldDIEUAIDE-NOUBHANI, Martine, ed., Ana Paula ALONSO, ed.. <i>Plant Metabolic Flux Analysis</i>. Totowa, NJ: Humana Press, 2014, pp. 281-299. Methods in Molecular Biology. 1090. ISBN 978-1-62703-687-0. Available under: doi: 10.1007/978-1-62703-688-7_17deu
kops.sourcefield.plainDIEUAIDE-NOUBHANI, Martine, ed., Ana Paula ALONSO, ed.. Plant Metabolic Flux Analysis. Totowa, NJ: Humana Press, 2014, pp. 281-299. Methods in Molecular Biology. 1090. ISBN 978-1-62703-687-0. Available under: doi: 10.1007/978-1-62703-688-7_17deu
kops.sourcefield.plainDIEUAIDE-NOUBHANI, Martine, ed., Ana Paula ALONSO, ed.. Plant Metabolic Flux Analysis. Totowa, NJ: Humana Press, 2014, pp. 281-299. Methods in Molecular Biology. 1090. ISBN 978-1-62703-687-0. Available under: doi: 10.1007/978-1-62703-688-7_17eng
relation.isAuthorOfPublication4a62a6c5-bf37-4efa-a633-4229ff88ed2e
relation.isAuthorOfPublication.latestForDiscovery4a62a6c5-bf37-4efa-a633-4229ff88ed2e
source.bibliographicInfo.fromPage281eng
source.bibliographicInfo.seriesNumber1090eng
source.bibliographicInfo.toPage299eng
source.contributor.editorDieuaide-Noubhani, Martine
source.contributor.editorAlonso, Ana Paula
source.identifier.isbn978-1-62703-687-0eng
source.publisherHumana Presseng
source.publisher.locationTotowa, NJeng
source.relation.ispartofseriesMethods in Molecular Biologyeng
source.titlePlant Metabolic Flux Analysiseng

Dateien