Publikation: Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy
Lade...
Dateien
Datum
2019
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
European Union (EU): 772027
Projekt
SPICE: Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes
Open Access-Veröffentlichung
Open Access Hybrid
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
ACS Chemical Biology. 2019, 14(5), pp. 839-844. ISSN 1554-8929. eISSN 1554-8937. Available under: doi: 10.1021/acschembio.8b01111
Zusammenfassung
Electron paramagnetic resonance spectroscopy in combination with site-directed spin labeling (SDSL) is an important tool to obtain long-range distance restraints for protein structural research. We here study a variety of azide- and alkyne-bearing noncanonical amino acids (ncAA) in terms of protein single- and double-incorporation efficiency via nonsense suppression, metabolic stability, yields of nitroxide labeling via copper-catalyzed [3 + 2] azide–alkyne cycloadditions (CuAAC), and spectroscopic properties in continuous-wave and double electron–electron resonance measurements. We identify para-ethynyl-l-phenylalanine and para-propargyloxy-l-phenylalanine as suitable ncAA for CuAAC-based SDSL that will complement current SDSL approaches, particularly in cases in which essential cysteines of a target protein prevent the use of sulfhydryl-reactive spin labels.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
WIDDER, Pia, Frederic BERNER, Daniel SUMMERER, Malte DRESCHER, 2019. Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy. In: ACS Chemical Biology. 2019, 14(5), pp. 839-844. ISSN 1554-8929. eISSN 1554-8937. Available under: doi: 10.1021/acschembio.8b01111BibTex
@article{Widder2019-05-17Doubl-45999,
year={2019},
doi={10.1021/acschembio.8b01111},
title={Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy},
number={5},
volume={14},
issn={1554-8929},
journal={ACS Chemical Biology},
pages={839--844},
author={Widder, Pia and Berner, Frederic and Summerer, Daniel and Drescher, Malte}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/45999">
<bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/45999"/>
<dc:contributor>Widder, Pia</dc:contributor>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/45999/1/Widder_2-zfywk5o2w02p4.pdf"/>
<dc:creator>Widder, Pia</dc:creator>
<dc:creator>Berner, Frederic</dc:creator>
<dc:contributor>Summerer, Daniel</dc:contributor>
<dc:creator>Drescher, Malte</dc:creator>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/45999/1/Widder_2-zfywk5o2w02p4.pdf"/>
<dc:contributor>Berner, Frederic</dc:contributor>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<dc:language>eng</dc:language>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-06-13T11:27:50Z</dcterms:available>
<dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
<dcterms:issued>2019-05-17</dcterms:issued>
<dcterms:abstract xml:lang="eng">Electron paramagnetic resonance spectroscopy in combination with site-directed spin labeling (SDSL) is an important tool to obtain long-range distance restraints for protein structural research. We here study a variety of azide- and alkyne-bearing noncanonical amino acids (ncAA) in terms of protein single- and double-incorporation efficiency via nonsense suppression, metabolic stability, yields of nitroxide labeling via copper-catalyzed [3 + 2] azide–alkyne cycloadditions (CuAAC), and spectroscopic properties in continuous-wave and double electron–electron resonance measurements. We identify para-ethynyl-l-phenylalanine and para-propargyloxy-l-phenylalanine as suitable ncAA for CuAAC-based SDSL that will complement current SDSL approaches, particularly in cases in which essential cysteines of a target protein prevent the use of sulfhydryl-reactive spin labels.</dcterms:abstract>
<dc:creator>Summerer, Daniel</dc:creator>
<dc:contributor>Drescher, Malte</dc:contributor>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-06-13T11:27:50Z</dc:date>
<dc:rights>terms-of-use</dc:rights>
<dcterms:title>Double Nitroxide Labeling by Copper-Catalyzed Azide–Alkyne Cycloadditions with Noncanonical Amino Acids for Electron Paramagnetic Resonance Spectroscopy</dcterms:title>
</rdf:Description>
</rdf:RDF>Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
