The dual aptamer approach : rational design of a high-affinity FAD aptamer

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dc.contributor.authorMerkle, Tobias
dc.contributor.authorHolder, Isabelle T.
dc.contributor.authorHartig, Jörg S.
dc.date.accessioned2015-12-04T09:35:18Z
dc.date.available2015-12-04T09:35:18Z
dc.date.issued2015-12-23
dc.description.abstractA design strategy for high-affinity aptamers of complex biomolecules is presented. We developed an RNA with FAD-binding properties by combining known ATP- and FMN-aptamers. Cooperative binding of FAD was shown by SPR spectroscopy and fluorescence assays. The strategy should be transferable to several other biomolecules.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1039/C5OB02026Ceng
dc.identifier.pmid26586417
dc.identifier.ppn454845456
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/32337
dc.language.isoengeng
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dc.subject.ddc540eng
dc.titleThe dual aptamer approach : rational design of a high-affinity FAD aptamereng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Merkle2015-12-23aptam-32337,
  year={2015},
  doi={10.1039/C5OB02026C},
  title={The dual aptamer approach : rational design of a high-affinity FAD aptamer},
  number={2},
  volume={14},
  issn={1477-0520},
  journal={Organic and Biomolecular Chemistry},
  pages={447--450},
  author={Merkle, Tobias and Holder, Isabelle T. and Hartig, Jörg S.}
}
kops.citation.iso690MERKLE, Tobias, Isabelle T. HOLDER, Jörg S. HARTIG, 2015. The dual aptamer approach : rational design of a high-affinity FAD aptamer. In: Organic and Biomolecular Chemistry. 2015, 14(2), pp. 447-450. ISSN 1477-0520. eISSN 1477-0539. Available under: doi: 10.1039/C5OB02026Cdeu
kops.citation.iso690MERKLE, Tobias, Isabelle T. HOLDER, Jörg S. HARTIG, 2015. The dual aptamer approach : rational design of a high-affinity FAD aptamer. In: Organic and Biomolecular Chemistry. 2015, 14(2), pp. 447-450. ISSN 1477-0520. eISSN 1477-0539. Available under: doi: 10.1039/C5OB02026Ceng
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