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A small-angle X-ray scattering study of red blood cells in continuous flow

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2023

Autor:innen

Burchert, Jan-Philipp
Graceffa, Rita
Burghammer, Manfred
Köster, Sarah

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Published

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Journal of Synchrotron Radiation. International Union of Crystallography (IUCr). 2023, 30(3), pp. 582-590. ISSN 0909-0495. eISSN 1600-5775. Available under: doi: 10.1107/s1600577523002011

Zusammenfassung

Owing to their large penetration depth and high resolution, X-rays are ideally suited to study structures and structural changes within intact biological cells. For this reason, X-ray-based techniques have been used to investigate adhesive cells on solid supports. However, these techniques cannot easily be transferred to the investigation of suspended cells in flow. Here, an X-ray compatible microfluidic device that serves as a sample delivery system and measurement environment for such studies is presented. As a proof of concept, the microfluidic device is applied to investigate chemically fixed bovine red blood cells by small-angle X-ray scattering (SAXS). A very good agreement is found between in-flow and static SAXS data. Moreover, the data are fitted with a hard-sphere model and screened Coulomb interactions to obtain the radius of the protein hemoglobin within the cells. Thus, the utility of this device for studying suspended cells with SAXS in continuous flow is demonstrated.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

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red blood cells, SAXS, microfluidics, cells in flow

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ISO 690BURCHERT, Jan-Philipp, Rita GRACEFFA, Oliva SALDANHA, Manfred BURGHAMMER, Sarah KÖSTER, 2023. A small-angle X-ray scattering study of red blood cells in continuous flow. In: Journal of Synchrotron Radiation. International Union of Crystallography (IUCr). 2023, 30(3), pp. 582-590. ISSN 0909-0495. eISSN 1600-5775. Available under: doi: 10.1107/s1600577523002011
BibTex
@article{Burchert2023small-67208,
  year={2023},
  doi={10.1107/s1600577523002011},
  title={A small-angle X-ray scattering study of red blood cells in continuous flow},
  number={3},
  volume={30},
  issn={0909-0495},
  journal={Journal of Synchrotron Radiation},
  pages={582--590},
  author={Burchert, Jan-Philipp and Graceffa, Rita and Saldanha, Oliva and Burghammer, Manfred and Köster, Sarah}
}
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