Excess labile carbon promotes the expression of virulence factors in coral reef bacterioplankton

Lade...
Vorschaubild
Dateien
Cárdenas_2-14nmphynia9552.pdf
Cárdenas_2-14nmphynia9552.pdfGröße: 4.76 MBDownloads: 97
Datum
2018
Autor:innen
Neave, Matthew J.
Haroon, Mohamed Fauzi
Wild, Christian
Gärdes, Astrid
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
EU-Projektnummer
DFG-Projektnummer
Projekt
Open Access-Veröffentlichung
Sammlungen
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
The ISME journal. Nature Publishing Group. 2018, 12(1), pp. 59-76. ISSN 1751-7362. eISSN 1751-7370. Available under: doi: 10.1038/ismej.2017.142
Zusammenfassung

Coastal pollution and algal cover are increasing on many coral reefs, resulting in higher dissolved organic carbon (DOC) concentrations. High DOC concentrations strongly affect microbial activity in reef waters and select for copiotrophic, often potentially virulent microbial populations. High DOC concentrations on coral reefs are also hypothesized to be a determinant for switching microbial lifestyles from commensal to pathogenic, thereby contributing to coral reef degradation, but evidence is missing. In this study, we conducted ex situ incubations to assess gene expression of planktonic microbial populations under elevated concentrations of naturally abundant monosaccharides (glucose, galactose, mannose, and xylose) in algal exudates and sewage inflows. We assembled 27 near-complete (>70%) microbial genomes through metagenomic sequencing and determined associated expression patterns through metatranscriptomic sequencing. Differential gene expression analysis revealed a shift in the central carbohydrate metabolism and the induction of metalloproteases, siderophores, and toxins in Alteromonas, Erythrobacter, Oceanicola, and Alcanivorax populations. Sugar-specific induction of virulence factors suggests a mechanistic link for the switch from a commensal to a pathogenic lifestyle, particularly relevant during increased algal cover and human-derived pollution on coral reefs. Although an explicit test remains to be performed, our data support the hypothesis that increased availability of specific sugars changes net microbial community activity in ways that increase the emergence and abundance of opportunistic pathogens, potentially contributing to coral reef degradation.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690CÁRDENAS, Anny, Matthew J. NEAVE, Mohamed Fauzi HAROON, Claudia POGOREUTZ, Nils RÄDECKER, Christian WILD, Astrid GÄRDES, Christian R. VOOLSTRA, 2018. Excess labile carbon promotes the expression of virulence factors in coral reef bacterioplankton. In: The ISME journal. Nature Publishing Group. 2018, 12(1), pp. 59-76. ISSN 1751-7362. eISSN 1751-7370. Available under: doi: 10.1038/ismej.2017.142
BibTex
@article{Cardenas2018Exces-51097,
  year={2018},
  doi={10.1038/ismej.2017.142},
  title={Excess labile carbon promotes the expression of virulence factors in coral reef bacterioplankton},
  number={1},
  volume={12},
  issn={1751-7362},
  journal={The ISME journal},
  pages={59--76},
  author={Cárdenas, Anny and Neave, Matthew J. and Haroon, Mohamed Fauzi and Pogoreutz, Claudia and Rädecker, Nils and Wild, Christian and Gärdes, Astrid and Voolstra, Christian R.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/51097">
    <dc:creator>Haroon, Mohamed Fauzi</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Gärdes, Astrid</dc:contributor>
    <dc:creator>Cárdenas, Anny</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/51097/1/C%c3%a1rdenas_2-14nmphynia9552.pdf"/>
    <dcterms:issued>2018</dcterms:issued>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/51097/1/C%c3%a1rdenas_2-14nmphynia9552.pdf"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:contributor>Haroon, Mohamed Fauzi</dc:contributor>
    <dc:creator>Rädecker, Nils</dc:creator>
    <dc:creator>Gärdes, Astrid</dc:creator>
    <dc:contributor>Wild, Christian</dc:contributor>
    <dc:creator>Voolstra, Christian R.</dc:creator>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-29T10:43:02Z</dcterms:available>
    <dc:creator>Wild, Christian</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/51097"/>
    <dcterms:abstract xml:lang="eng">Coastal pollution and algal cover are increasing on many coral reefs, resulting in higher dissolved organic carbon (DOC) concentrations. High DOC concentrations strongly affect microbial activity in reef waters and select for copiotrophic, often potentially virulent microbial populations. High DOC concentrations on coral reefs are also hypothesized to be a determinant for switching microbial lifestyles from commensal to pathogenic, thereby contributing to coral reef degradation, but evidence is missing. In this study, we conducted ex situ incubations to assess gene expression of planktonic microbial populations under elevated concentrations of naturally abundant monosaccharides (glucose, galactose, mannose, and xylose) in algal exudates and sewage inflows. We assembled 27 near-complete (&gt;70%) microbial genomes through metagenomic sequencing and determined associated expression patterns through metatranscriptomic sequencing. Differential gene expression analysis revealed a shift in the central carbohydrate metabolism and the induction of metalloproteases, siderophores, and toxins in Alteromonas, Erythrobacter, Oceanicola, and Alcanivorax populations. Sugar-specific induction of virulence factors suggests a mechanistic link for the switch from a commensal to a pathogenic lifestyle, particularly relevant during increased algal cover and human-derived pollution on coral reefs. Although an explicit test remains to be performed, our data support the hypothesis that increased availability of specific sugars changes net microbial community activity in ways that increase the emergence and abundance of opportunistic pathogens, potentially contributing to coral reef degradation.</dcterms:abstract>
    <dc:contributor>Cárdenas, Anny</dc:contributor>
    <dc:contributor>Neave, Matthew J.</dc:contributor>
    <dcterms:title>Excess labile carbon promotes the expression of virulence factors in coral reef bacterioplankton</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-09-29T10:43:02Z</dc:date>
    <dc:contributor>Voolstra, Christian R.</dc:contributor>
    <dc:creator>Neave, Matthew J.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Pogoreutz, Claudia</dc:contributor>
    <dc:creator>Pogoreutz, Claudia</dc:creator>
    <dc:contributor>Rädecker, Nils</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Ja