Publikation:

Assembly of a unique membrane complex in type VI secretion systems of Bacteroidota

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2024

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Bongiovanni, Thibault R.
Latario, Casey J.
Le Cras, Youn
Trus, Evan
Robitaille, Sophie
Swartz, Kerry
Ross, Benjamin D.
Durand, Eric
et al.

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Nature Communications. Springer. 2024, 15, 429. eISSN 2041-1723. Available under: doi: 10.1038/s41467-023-44426-1

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The type VI secretion system (T6SS) of Gram-negative bacteria inhibits competitor cells through contact-dependent translocation of toxic effector proteins. In Proteobacteria, the T6SS is anchored to the cell envelope through a megadalton-sized membrane complex (MC). However, the genomes of Bacteroidota with T6SSs appear to lack genes encoding homologs of canonical MC components. Here, we identify five genes in Bacteroides fragilis (tssNQOPR) that are essential for T6SS function and encode a Bacteroidota-specific MC. We purify this complex, reveal its dimensions using electron microscopy, and identify a protein-protein interaction network underlying the assembly of the MC including the stoichiometry of the five TssNQOPR components. Protein TssN mediates the connection between the Bacteroidota MC and the conserved baseplate. Although MC gene content and organization varies across the phylum Bacteroidota, no MC homologs are detected outside of T6SS loci, suggesting ancient co-option and functional convergence with the non-homologous MC of Pseudomonadota.

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570 Biowissenschaften, Biologie

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ISO 690BONGIOVANNI, Thibault R., Casey J. LATARIO, Youn LE CRAS, Evan TRUS, Sophie ROBITAILLE, Kerry SWARTZ, Jan ORTH, Florian STENGEL, Benjamin D. ROSS, Eric DURAND, 2024. Assembly of a unique membrane complex in type VI secretion systems of Bacteroidota. In: Nature Communications. Springer. 2024, 15, 429. eISSN 2041-1723. Available under: doi: 10.1038/s41467-023-44426-1
BibTex
@article{Bongiovanni2024Assem-69335,
  year={2024},
  doi={10.1038/s41467-023-44426-1},
  title={Assembly of a unique membrane complex in type VI secretion systems of Bacteroidota},
  volume={15},
  journal={Nature Communications},
  author={Bongiovanni, Thibault R. and Latario, Casey J. and Le Cras, Youn and Trus, Evan and Robitaille, Sophie and Swartz, Kerry and Orth, Jan and Stengel, Florian and Ross, Benjamin D. and Durand, Eric},
  note={Article Number: 429}
}
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