Backbone Structure of Transmembrane Domain IX of the Na+/Proline Transporter PutP of Escherichia coli

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2009
Autor:innen
Hilger, Daniel
Polyhach, Yevhen
Jung, Heinrich
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
EU-Projektnummer
DFG-Projektnummer
Angaben zur Forschungsförderung (Freitext)
Projekt
Open Access-Veröffentlichung
Sammlungen
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Biophysical Journal. Cell Press. 2009, 96(1), pp. 217-225. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2008.09.030
Zusammenfassung

The backbone structure is determined by site-directed spin labeling, double electron electron resonance measurements of distances, and modeling in terms of a helix-loop-helix construct for a transmembrane domain that is supposed to line the translocation pathway in the 54.3 kDa Na+/proline symporter PutP of Escherichia coli. The conformational distribution of the spin labels is accounted for by a rotamer library. An ensemble of backbone models with a root mean-square deviation of less than 2 Å is obtained. These models exhibit a pronounced kink near residue T341, which is involved in substrate binding. The kink may be associated with a hinge that allows the protein to open and close an inwardly oriented cavity.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690HILGER, Daniel, Yevhen POLYHACH, Heinrich JUNG, Gunnar JESCHKE, 2009. Backbone Structure of Transmembrane Domain IX of the Na+/Proline Transporter PutP of Escherichia coli. In: Biophysical Journal. Cell Press. 2009, 96(1), pp. 217-225. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2008.09.030
BibTex
@article{Hilger2009-01Backb-58510,
  year={2009},
  doi={10.1016/j.bpj.2008.09.030},
  title={Backbone Structure of Transmembrane Domain IX of the Na<sup>+</sup>/Proline Transporter PutP of Escherichia coli},
  number={1},
  volume={96},
  issn={0006-3495},
  journal={Biophysical Journal},
  pages={217--225},
  author={Hilger, Daniel and Polyhach, Yevhen and Jung, Heinrich and Jeschke, Gunnar}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/58510">
    <dc:contributor>Jung, Heinrich</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-06T13:30:18Z</dcterms:available>
    <dcterms:abstract xml:lang="eng">The backbone structure is determined by site-directed spin labeling, double electron electron resonance measurements of distances, and modeling in terms of a helix-loop-helix construct for a transmembrane domain that is supposed to line the translocation pathway in the 54.3 kDa Na&lt;sup&gt;+&lt;/sup&gt;/proline symporter PutP of Escherichia coli. The conformational distribution of the spin labels is accounted for by a rotamer library. An ensemble of backbone models with a root mean-square deviation of less than 2 Å is obtained. These models exhibit a pronounced kink near residue T341, which is involved in substrate binding. The kink may be associated with a hinge that allows the protein to open and close an inwardly oriented cavity.</dcterms:abstract>
    <dc:contributor>Polyhach, Yevhen</dc:contributor>
    <dcterms:title>Backbone Structure of Transmembrane Domain IX of the Na&lt;sup&gt;+&lt;/sup&gt;/Proline Transporter PutP of Escherichia coli</dcterms:title>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Polyhach, Yevhen</dc:creator>
    <dcterms:issued>2009-01</dcterms:issued>
    <dc:contributor>Jeschke, Gunnar</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/58510"/>
    <dc:creator>Jung, Heinrich</dc:creator>
    <dc:contributor>Hilger, Daniel</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-06T13:30:18Z</dc:date>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:language>eng</dc:language>
    <dc:creator>Hilger, Daniel</dc:creator>
    <dc:creator>Jeschke, Gunnar</dc:creator>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Ja
Diese Publikation teilen