Backbone Structure of Transmembrane Domain IX of the Na+/Proline Transporter PutP of Escherichia coli
| dc.contributor.author | Hilger, Daniel | |
| dc.contributor.author | Polyhach, Yevhen | |
| dc.contributor.author | Jung, Heinrich | |
| dc.contributor.author | Jeschke, Gunnar | |
| dc.date.accessioned | 2022-09-06T13:30:18Z | |
| dc.date.available | 2022-09-06T13:30:18Z | |
| dc.date.issued | 2009-01 | eng |
| dc.description.abstract | The backbone structure is determined by site-directed spin labeling, double electron electron resonance measurements of distances, and modeling in terms of a helix-loop-helix construct for a transmembrane domain that is supposed to line the translocation pathway in the 54.3 kDa Na+/proline symporter PutP of Escherichia coli. The conformational distribution of the spin labels is accounted for by a rotamer library. An ensemble of backbone models with a root mean-square deviation of less than 2 Å is obtained. These models exhibit a pronounced kink near residue T341, which is involved in substrate binding. The kink may be associated with a hinge that allows the protein to open and close an inwardly oriented cavity. | eng |
| dc.description.version | published | eng |
| dc.identifier.doi | 10.1016/j.bpj.2008.09.030 | eng |
| dc.identifier.pmid | 19134477 | eng |
| dc.identifier.uri | https://kops.uni-konstanz.de/handle/123456789/58510 | |
| dc.language.iso | eng | eng |
| dc.subject.ddc | 540 | eng |
| dc.title | Backbone Structure of Transmembrane Domain IX of the Na<sup>+</sup>/Proline Transporter PutP of Escherichia coli | eng |
| dc.type | JOURNAL_ARTICLE | eng |
| dspace.entity.type | Publication | |
| kops.citation.bibtex | @article{Hilger2009-01Backb-58510,
year={2009},
doi={10.1016/j.bpj.2008.09.030},
title={Backbone Structure of Transmembrane Domain IX of the Na<sup>+</sup>/Proline Transporter PutP of Escherichia coli},
number={1},
volume={96},
issn={0006-3495},
journal={Biophysical Journal},
pages={217--225},
author={Hilger, Daniel and Polyhach, Yevhen and Jung, Heinrich and Jeschke, Gunnar}
} | |
| kops.citation.iso690 | HILGER, Daniel, Yevhen POLYHACH, Heinrich JUNG, Gunnar JESCHKE, 2009. Backbone Structure of Transmembrane Domain IX of the Na+/Proline Transporter PutP of Escherichia coli. In: Biophysical Journal. Cell Press. 2009, 96(1), pp. 217-225. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2008.09.030 | deu |
| kops.citation.iso690 | HILGER, Daniel, Yevhen POLYHACH, Heinrich JUNG, Gunnar JESCHKE, 2009. Backbone Structure of Transmembrane Domain IX of the Na+/Proline Transporter PutP of Escherichia coli. In: Biophysical Journal. Cell Press. 2009, 96(1), pp. 217-225. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2008.09.030 | eng |
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| kops.sourcefield | Biophysical Journal. Cell Press. 2009, <b>96</b>(1), pp. 217-225. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2008.09.030 | deu |
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| kops.sourcefield.plain | Biophysical Journal. Cell Press. 2009, 96(1), pp. 217-225. ISSN 0006-3495. eISSN 1542-0086. Available under: doi: 10.1016/j.bpj.2008.09.030 | eng |
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