Publikation: Endocytosis and Membrane Turnover in the Germ Tube of Uromyces fabae
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
We have used the fluorescent dye FM4-64 as a tracer to demonstrate bulk membrane internalization (endocytosis) and redistribution of the dye within the cytoplasm of the germ tube of the rust fungus Uromyces fabae. Staining of the hyphal membrane was detected 4 s after application of FM4-64 and reached a maximum after 1 min. The highest fluorescence intensity occurred in the apex. Subsequently, staining of the plasma membrane decreased and a subapical region of the fungal protoplast (5 20 µm from the tip) displayed increasing fluorescence with a maximum after 5 min. Fluorescence in the subapical region was redistributed to an area in the hyphal tip, which corresponds to the accumulation of apical vesicles, after 10 15 min and subsequently to a cytoplasmic region in front of the two nuclei (35 45 µm from the tip). We conclude from our measurements of membrane fluorescence that the turnover time from endocytosis to secretion of the dye amounts to 15 min. The uptake of the dye into the cytoplasm, but not membrane loading, could be inhibited completely with 5 mM NaN3 or by a temperature shift to 4°C. This is the first evidence for endocytosis in a fungal germ tube.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
HOFFMANN, Jochen, Kurt MENDGEN, 1998. Endocytosis and Membrane Turnover in the Germ Tube of Uromyces fabae. In: Fungal Genetics and Biology. 1998, 24(1-2), pp. 77-85. ISSN 1087-1845. eISSN 1096-0937. Available under: doi: 10.1006/fgbi.1998.1059BibTex
@article{Hoffmann1998Endoc-7631, year={1998}, doi={10.1006/fgbi.1998.1059}, title={Endocytosis and Membrane Turnover in the Germ Tube of Uromyces fabae}, number={1-2}, volume={24}, issn={1087-1845}, journal={Fungal Genetics and Biology}, pages={77--85}, author={Hoffmann, Jochen and Mendgen, Kurt} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7631"> <dc:language>eng</dc:language> <dcterms:issued>1998</dcterms:issued> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:55Z</dcterms:available> <dc:creator>Hoffmann, Jochen</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7631"/> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:format>application/pdf</dc:format> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Mendgen, Kurt</dc:contributor> <dc:contributor>Hoffmann, Jochen</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7631/1/169_hoffmann_98.pdf"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:35:55Z</dc:date> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dc:creator>Mendgen, Kurt</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7631/1/169_hoffmann_98.pdf"/> <dcterms:bibliographicCitation>First publ. in: Fungal Genetics and Biology 24 (1998), pp. 77-85</dcterms:bibliographicCitation> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dcterms:abstract xml:lang="eng">We have used the fluorescent dye FM4-64 as a tracer to demonstrate bulk membrane internalization (endocytosis) and redistribution of the dye within the cytoplasm of the germ tube of the rust fungus Uromyces fabae. Staining of the hyphal membrane was detected 4 s after application of FM4-64 and reached a maximum after 1 min. The highest fluorescence intensity occurred in the apex. Subsequently, staining of the plasma membrane decreased and a subapical region of the fungal protoplast (5 20 µm from the tip) displayed increasing fluorescence with a maximum after 5 min. Fluorescence in the subapical region was redistributed to an area in the hyphal tip, which corresponds to the accumulation of apical vesicles, after 10 15 min and subsequently to a cytoplasmic region in front of the two nuclei (35 45 µm from the tip). We conclude from our measurements of membrane fluorescence that the turnover time from endocytosis to secretion of the dye amounts to 15 min. The uptake of the dye into the cytoplasm, but not membrane loading, could be inhibited completely with 5 mM NaN3 or by a temperature shift to 4°C. This is the first evidence for endocytosis in a fungal germ tube.</dcterms:abstract> <dcterms:title>Endocytosis and Membrane Turnover in the Germ Tube of Uromyces fabae</dcterms:title> </rdf:Description> </rdf:RDF>