6-Substituted 2-Aminopurine-2′-deoxyribonucleoside 5′-Triphosphates that Trace Cytosine Methylation

Kurzanzeige
dc.contributor.authorvon Watzdorf, Janina
dc.contributor.authorMarx, Andreas
dc.date.accessioned2017-02-14T11:26:16Z
dc.date.available2017-02-14T11:26:16Z
dc.date.issued2016eng
dc.description.abstractGene expression is extensively regulated by the occurrence and distribution of the epigenetic marker 2'-deoxy 5-methylcytosine (5mC) in genomic DNA. Because of its effects on tumorigenesis there is an important link to human health. In addition, detection of 5mC can serve as an outstanding biomarker for diagnostics as well as for disease therapy. Our previous studies have already shown that, by processing O(6) -alkylated 2'-deoxyguanosine triphosphate (dGTP) analogues, DNA polymerases are able to sense the presence of a single 5mC unit in a template. Here we present the synthesis and evaluation of an extended toolbox of 6-substituted 2-aminopurine-2'-deoxyribonucleoside 5'-triphosphates modified at position 6 with various functionalities. We found that sensing of 5-methylation by this class of nucleotides is more general, not being restricted to O(6) -alkyl modification of dGTP but also applying to other functionalities.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1002/cbic.201600245eng
dc.identifier.pmid27253512eng
dc.identifier.ppn48343129X
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/37407
dc.language.isoengeng
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc540eng
dc.title6-Substituted 2-Aminopurine-2′-deoxyribonucleoside 5′-Triphosphates that Trace Cytosine Methylationeng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{vonWatzdorf20166Subs-37407,
  year={2016},
  doi={10.1002/cbic.201600245},
  title={6-Substituted 2-Aminopurine-2′-deoxyribonucleoside 5′-Triphosphates that Trace Cytosine Methylation},
  number={16},
  volume={17},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={1532--1540},
  author={von Watzdorf, Janina and Marx, Andreas}
}
kops.citation.iso690VON WATZDORF, Janina, Andreas MARX, 2016. 6-Substituted 2-Aminopurine-2′-deoxyribonucleoside 5′-Triphosphates that Trace Cytosine Methylation. In: ChemBioChem. 2016, 17(16), pp. 1532-1540. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201600245deu
kops.citation.iso690VON WATZDORF, Janina, Andreas MARX, 2016. 6-Substituted 2-Aminopurine-2′-deoxyribonucleoside 5′-Triphosphates that Trace Cytosine Methylation. In: ChemBioChem. 2016, 17(16), pp. 1532-1540. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201600245eng
kops.citation.rdf
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/37407">
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:issued>2016</dcterms:issued>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:title>6-Substituted 2-Aminopurine-2′-deoxyribonucleoside 5′-Triphosphates that Trace Cytosine Methylation</dcterms:title>
    <dc:language>eng</dc:language>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:creator>von Watzdorf, Janina</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/37407"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/37407/3/Watzdorf_0-369910.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-02-14T11:26:16Z</dc:date>
    <dc:rights>Attribution-NonCommercial-NoDerivatives 4.0 International</dc:rights>
    <dc:contributor>von Watzdorf, Janina</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/4.0/"/>
    <dc:creator>Marx, Andreas</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/37407/3/Watzdorf_0-369910.pdf"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:abstract xml:lang="eng">Gene expression is extensively regulated by the occurrence and distribution of the epigenetic marker 2'-deoxy 5-methylcytosine (5mC) in genomic DNA. Because of its effects on tumorigenesis there is an important link to human health. In addition, detection of 5mC can serve as an outstanding biomarker for diagnostics as well as for disease therapy. Our previous studies have already shown that, by processing O(6) -alkylated 2'-deoxyguanosine triphosphate (dGTP) analogues, DNA polymerases are able to sense the presence of a single 5mC unit in a template. Here we present the synthesis and evaluation of an extended toolbox of 6-substituted 2-aminopurine-2'-deoxyribonucleoside 5'-triphosphates modified at position 6 with various functionalities. We found that sensing of 5-methylation by this class of nucleotides is more general, not being restricted to O(6) -alkyl modification of dGTP but also applying to other functionalities.</dcterms:abstract>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-02-14T11:26:16Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>
kops.description.funding{"first": "eu", "second": "339834"}
kops.description.openAccessopenaccesshybrideng
kops.flag.knbibliographytrue
kops.identifier.nbnurn:nbn:de:bsz:352-0-369910
kops.relation.euProjectID339834
kops.relation.uniknProjectTitleEvoEPIGEN - Evolved Replication Systems of Epigenetics
kops.sourcefieldChemBioChem. 2016, <b>17</b>(16), pp. 1532-1540. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201600245deu
kops.sourcefield.plainChemBioChem. 2016, 17(16), pp. 1532-1540. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201600245deu
kops.sourcefield.plainChemBioChem. 2016, 17(16), pp. 1532-1540. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201600245eng
relation.isAuthorOfPublicationc5028888-f9b2-4f50-b93c-eb3855e8f3ef
relation.isAuthorOfPublication3488d192-4e21-4a69-8956-f7d02d9f9b3a
relation.isAuthorOfPublication.latestForDiscoveryc5028888-f9b2-4f50-b93c-eb3855e8f3ef
source.bibliographicInfo.fromPage1532eng
source.bibliographicInfo.issue16eng
source.bibliographicInfo.toPage1540eng
source.bibliographicInfo.volume17eng
source.identifier.eissn1439-7633eng
source.identifier.issn1439-4227eng
source.periodicalTitleChemBioChemeng

Dateien

Originalbündel

Gerade angezeigt 1 - 1 von 1
Vorschaubild nicht verfügbar
Name:
Watzdorf_0-369910.pdf
Größe:
1.57 MB
Format:
Adobe Portable Document Format
Beschreibung:
Watzdorf_0-369910.pdf
Watzdorf_0-369910.pdfGröße: 1.57 MBDownloads: 346

Lizenzbündel

Gerade angezeigt 1 - 1 von 1
Vorschaubild nicht verfügbar
Name:
license.txt
Größe:
3.88 KB
Format:
Item-specific license agreed upon to submission
Beschreibung:
license.txt
license.txtGröße: 3.88 KBDownloads: 0

Sammlungen

Chemie: Publikationen