Strategy to replace animal-derived ECM by a modular and highly defined matrix

Nesterenko, Yevheniia; Dolde, Xenia; Leist, Marcel; Mayans, Olga

Strategy to replace animal-derived ECM by a modular and highly defined matrix

Dateien

Nesterenko_2-1fumuhyjqgls41.pdfGröße: 2.26 MBDownloads: 265

Datum

2020

Autor:innen

Nesterenko, Yevheniia

Dolde, Xenia

Leist, Marcel

Mayans, Olga

EU-Projektnummer

681002

Projekt

EUToxRisk21

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Alternatives to Animal Experimentation : ALTEX. Springer Spektrum. 2020, 37(3), pp. 482-489. ISSN 1868-596X. eISSN 1868-8551. Available under: doi: 10.14573/altex.2003181

Zusammenfassung

Many extracellular matrices (ECM) used for modern cell culture are derived from animals. An alternative approach is the recombinant production of individual matrix protein components. A further development of this strategy uses a constant core protein polymer that is modifiable with functional domains of various ECM proteins. This way, a single, highly defined ECM system could be used for a large variety of cell types. Self-assembling protein domains from human muscle sarcomeres, termed here ZT material (ZT), have been shown to be suitable for this modular approach of generating ECMs. We explored in a proof-of-concept study, whether ZT, modified with the fibronectin 10 domain (ZTFn10) is able to substitute bovine serum-derived fibronectin as coating for neural crest cell (NCC)-based toxicity testing. Human NCC were generated from pluripotent stem cells and used in the automated version of a NCC migration assay (cMINC). ZTFn10, but not the unmodified core material (ZT), allowed for a high migration activity. The classical cMINC setup, with bovine fibronectin coating, was used as positive control, and detailed analysis of NCC migration by time-lapse recording indicated that the novel ECM fully matched the bioactivity of the traditional ECM. A final set of experiments showed that various positive controls of the cMINC assay (PCB180, LiCl, cytochalasin D) showed nearly identical inhibition curves on the traditional and the novel ECM. Thus, the cMINC, and possibly other bioassays, can be performed with a ZT-based ECM instead of traditional animal-derived protein coatings.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

NESTERENKO, Yevheniia, Xenia DOLDE, Marcel LEIST, Olga MAYANS, 2020. Strategy to replace animal-derived ECM by a modular and highly defined matrix. In: Alternatives to Animal Experimentation : ALTEX. Springer Spektrum. 2020, 37(3), pp. 482-489. ISSN 1868-596X. eISSN 1868-8551. Available under: doi: 10.14573/altex.2003181

BibTex

@article{Nesterenko2020Strat-49891,
  year={2020},
  doi={10.14573/altex.2003181},
  title={Strategy to replace animal-derived ECM by a modular and highly defined matrix},
  number={3},
  volume={37},
  issn={1868-596X},
  journal={Alternatives to Animal Experimentation : ALTEX},
  pages={482--489},
  author={Nesterenko, Yevheniia and Dolde, Xenia and Leist, Marcel and Mayans, Olga}
}

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