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Anle138b interaction in α-synuclein aggregates by dynamic nuclear polarization NMR

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2023

Autor:innen

Dervişoğlu, Rıza
Antonschmidt, Leif
Nimerovsky, Evgeny
Sant, Vrinda
Kim, Myeongkyu
Ryazanov, Sergey
Leonov, Andrei
Fuentes-Monteverde, Juan Carlos
Andreas, Loren B.
et al.

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Published

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Methods. Elsevier. 2023, 214, pp. 18-27. ISSN 1046-2023. eISSN 1095-9130. Available under: doi: 10.1016/j.ymeth.2023.04.002

Zusammenfassung

Small molecules that bind to oligomeric protein species such as membrane proteins and fibrils are of clinical interest for development of therapeutics and diagnostics. Definition of the binding site at atomic resolution via NMR is often challenging due to low binding stoichiometry of the small molecule. For fibrils and aggregation intermediates grown in the presence of lipids, we report atomic-resolution contacts to the small molecule at sub nm distance via solid-state NMR using dynamic nuclear polarization (DNP) and orthogonally labelled samples of the protein and the small molecule. We apply this approach to α-synuclein (αS) aggregates in complex with the small molecule anle138b, which is a clinical drug candidate for disease modifying therapy. The small central pyrazole moiety of anle138b is detected in close proximity to the protein backbone and differences in the contacts between fibrils and early intermediates are observed. For intermediate species, the 100 K condition for DNP helps to preserve the aggregation state, while for both fibrils and oligomers, the DNP enhancement is essential to obtain sufficient sensitivity.

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Fachgebiet (DDC)
540 Chemie

Schlagwörter

amyloid, membrane-protein, DNP-NMR, MAS-NMR, drug, small-molecule, atomic distance, anle138b, alpha- synuclein

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ISO 690DERVIŞOĞLU, Rıza, Leif ANTONSCHMIDT, Evgeny NIMEROVSKY, Vrinda SANT, Myeongkyu KIM, Sergey RYAZANOV, Andrei LEONOV, Juan Carlos FUENTES-MONTEVERDE, Guinevere MATHIES, Loren B. ANDREAS, 2023. Anle138b interaction in α-synuclein aggregates by dynamic nuclear polarization NMR. In: Methods. Elsevier. 2023, 214, pp. 18-27. ISSN 1046-2023. eISSN 1095-9130. Available under: doi: 10.1016/j.ymeth.2023.04.002
BibTex
@article{Dervisoglu2023Anle1-67455,
  year={2023},
  doi={10.1016/j.ymeth.2023.04.002},
  title={Anle138b interaction in α-synuclein aggregates by dynamic nuclear polarization NMR},
  volume={214},
  issn={1046-2023},
  journal={Methods},
  pages={18--27},
  author={Dervişoğlu, Rıza and Antonschmidt, Leif and Nimerovsky, Evgeny and Sant, Vrinda and Kim, Myeongkyu and Ryazanov, Sergey and Leonov, Andrei and Fuentes-Monteverde, Juan Carlos and Mathies, Guinevere and Andreas, Loren B.}
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