@article{Barreras2022-02human-57023,
  year={2022},
  doi={10.1007/s13365-022-01062-7},
  title={A human-derived 3D brain organoid model to study JC virus infection},
  number={1},
  volume={28},
  issn={1355-0284},
  journal={Journal of neurovirology},
  pages={17--26},
  author={Barreras, Paula and Pamies, David and Monaco, Maria Chiara and Muñoz, Laura S. and Zhong, Xiali and Major, Eugene O. and Hogberg, Helena T. and Hartung, Thomas and Pardo, Carlos A.}
}

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    <dc:contributor>Zhong, Xiali</dc:contributor>
    <dc:contributor>Hartung, Thomas</dc:contributor>
    <dc:contributor>Monaco, Maria Chiara</dc:contributor>
    <dc:creator>Monaco, Maria Chiara</dc:creator>
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    <dc:creator>Hartung, Thomas</dc:creator>
    <dc:creator>Muñoz, Laura S.</dc:creator>
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    <dc:language>eng</dc:language>
    <dc:contributor>Hogberg, Helena T.</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-28T15:17:14Z</dcterms:available>
    <dc:creator>Zhong, Xiali</dc:creator>
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    <dc:contributor>Barreras, Paula</dc:contributor>
    <dc:contributor>Pardo, Carlos A.</dc:contributor>
    <dc:contributor>Pamies, David</dc:contributor>
    <dc:creator>Pamies, David</dc:creator>
    <dcterms:abstract xml:lang="eng">Progressive multifocal leukoencephalopathy (PML) is a frequent neurological complication in immunosuppressed patients. PML is caused by the JC virus (JCV), a neurotropic DNA polyomavirus that infects oligodendrocytes and astrocytes, causing inflammation and demyelination which lead to neurological dysfunction. The pathogenesis of PML is poorly understood due to the lack of in vitro or animal models to study mechanisms of disease as the virus most efficiently infects only human cells. We developed a human-derived brain organotypic system (also called brain organoid) to model JCV infection. The model was developed by using human-induced pluripotent stem cells (iPSC) and culturing them in 3D to generate an organotypic model containing neurons, astrocytes, and oligodendrocytes which recapitulates aspects of the environment of the human brain. We infected the brain organoids with the JCV MAD4 strain or cerebrospinal fluid of a patient with PML. The organoids were assessed for evidence of infection by qPCR, immunofluorescence, and electron microscopy at 1, 2, and 3 weeks post-exposure. JCV infection in both JCV MAD4 strain and PML CSF-exposed brain organoids was confirmed by immunocytochemical studies demonstrating viral antigens and electron microscopy showing virion particles in the nuclear compartment of oligodendrocytes and astrocytes. No evidence of neuronal infection was visualized. Infection was also demonstrated by JCV qPCR in the virus-exposed organoids and their media. In conclusion, the brain organoid model of JCV infection establishes a human model suitable for studying the mechanisms of JCV infection and pathogenesis of PML and may facilitate the exploration of therapeutic approaches.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-03-28T15:17:14Z</dc:date>
    <dc:creator>Major, Eugene O.</dc:creator>
    <dc:creator>Barreras, Paula</dc:creator>
    <dcterms:issued>2022-02</dcterms:issued>
    <dc:contributor>Muñoz, Laura S.</dc:contributor>
    <dc:contributor>Major, Eugene O.</dc:contributor>
    <dc:creator>Hogberg, Helena T.</dc:creator>
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    <dcterms:title>A human-derived 3D brain organoid model to study JC virus infection</dcterms:title>
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    <dc:creator>Pardo, Carlos A.</dc:creator>
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