Publikation: Dichloromethane utilized by an anaerobic mixed culture: acetogenesis and methanogenesis
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Dichloromethane (8.9 mg/l) was eliminated from industrially polluted, anaerobic groundwater in a fixed-bed reactor (43 m3) which was packed with activated charcoal and operated continuously for over three years. The elimination of dichloromethane over this period was some ten-fold in excess of the sorptive capacity of the charcoal, and the elimination (3.7 mg/h·[kg of charcoal]: residence time, 49 h) was tentatively attributed to dehalogenative microorganisms immobilized on the charcoal. Anaerobic enrichment cultures, with dichloromethane as the sole added source of carbon and energy, were inoculated with material from the reactor. Reproducibly complete substrate disappearance in subcultures was observed when traces of groundwater (1%) or yeast extract (0.01%) were supplied. Fed-batch experiments under an atmosphere of CO2 plus N2 led to the conversion in 11 days of 11 mM dichloromethane to 3 mM acetate and 2 mM methane, with a growth yield of 0.4 g of protein/mol of dichloromethane; insignificant amounts (
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STROMEYER, Susanna A., Wolfgang WINKELBAUER, Herbert KOHLER, Alasdair M. COOK, 1991. Dichloromethane utilized by an anaerobic mixed culture: acetogenesis and methanogenesis. In: Biodegradation. 1991, 2(2), pp. 129-137. ISSN 0923-9820. eISSN 1572-9729. Available under: doi: 10.1007/BF00114603BibTex
@article{Stromeyer1991Dichl-7407, year={1991}, doi={10.1007/BF00114603}, title={Dichloromethane utilized by an anaerobic mixed culture: acetogenesis and methanogenesis}, number={2}, volume={2}, issn={0923-9820}, journal={Biodegradation}, pages={129--137}, author={Stromeyer, Susanna A. and Winkelbauer, Wolfgang and Kohler, Herbert and Cook, Alasdair M.} }
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