Publikation: In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells
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Datum
2001
Autor:innen
Hentze, Hannes
Schwoebel, Frank
Lund, Søren
Kehl, Marius
Ertel, Wolfgang
Jäättelä, Marja
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Biochemical and Biophysical Research Communications. Elsevier. 2001, 283(5), pp. 1111-1117. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.2001.4918
Zusammenfassung
While caspases play an established role as intracellular executors of apoptosis, little is known about extracellular activities of this ubiquitously expressed family of proteases. We demonstrate here that recombinant caspase-3 retained enzymatic activity in various extracellular fluids. Experiments with cell lines, primary cells, and mice with fulminant CD95-triggered hepatitis showed that significant amounts of DEVD-aminofluoromethylcoumarine-cleaving activity, indicative of active effector caspases, were released into the medium/plasma during apoptosis. Furthermore, caspase activities were detected in liquor samples from human head trauma patients. These findings warrant closer investigation of DEVDase activity as a diagnostic marker, and of potential extracellular substrates for caspases.
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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
apoptosis, necrosis, caspase, liver, redox, protease assay, plasma, serum, extracellular
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HENTZE, Hannes, Frank SCHWOEBEL, Søren LUND, Marius KEHL, Wolfgang ERTEL, Albrecht WENDEL, Marja JÄÄTTELÄ, Marcel LEIST, 2001. In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells. In: Biochemical and Biophysical Research Communications. Elsevier. 2001, 283(5), pp. 1111-1117. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.2001.4918BibTex
@article{Hentze2001-05-25vitro-51266,
year={2001},
doi={10.1006/bbrc.2001.4918},
title={In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells},
number={5},
volume={283},
issn={0006-291X},
journal={Biochemical and Biophysical Research Communications},
pages={1111--1117},
author={Hentze, Hannes and Schwoebel, Frank and Lund, Søren and Kehl, Marius and Ertel, Wolfgang and Wendel, Albrecht and Jäättelä, Marja and Leist, Marcel}
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<dcterms:abstract xml:lang="eng">While caspases play an established role as intracellular executors of apoptosis, little is known about extracellular activities of this ubiquitously expressed family of proteases. We demonstrate here that recombinant caspase-3 retained enzymatic activity in various extracellular fluids. Experiments with cell lines, primary cells, and mice with fulminant CD95-triggered hepatitis showed that significant amounts of DEVD-aminofluoromethylcoumarine-cleaving activity, indicative of active effector caspases, were released into the medium/plasma during apoptosis. Furthermore, caspase activities were detected in liquor samples from human head trauma patients. These findings warrant closer investigation of DEVDase activity as a diagnostic marker, and of potential extracellular substrates for caspases.</dcterms:abstract>
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