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In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells

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2001

Autor:innen

Hentze, Hannes
Schwoebel, Frank
Lund, Søren
Kehl, Marius
Ertel, Wolfgang
Jäättelä, Marja

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Core Facility der Universität Konstanz

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Biochemical and Biophysical Research Communications. Elsevier. 2001, 283(5), pp. 1111-1117. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.2001.4918

Zusammenfassung

While caspases play an established role as intracellular executors of apoptosis, little is known about extracellular activities of this ubiquitously expressed family of proteases. We demonstrate here that recombinant caspase-3 retained enzymatic activity in various extracellular fluids. Experiments with cell lines, primary cells, and mice with fulminant CD95-triggered hepatitis showed that significant amounts of DEVD-aminofluoromethylcoumarine-cleaving activity, indicative of active effector caspases, were released into the medium/plasma during apoptosis. Furthermore, caspase activities were detected in liquor samples from human head trauma patients. These findings warrant closer investigation of DEVDase activity as a diagnostic marker, and of potential extracellular substrates for caspases.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

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apoptosis, necrosis, caspase, liver, redox, protease assay, plasma, serum, extracellular

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ISO 690HENTZE, Hannes, Frank SCHWOEBEL, Søren LUND, Marius KEHL, Wolfgang ERTEL, Albrecht WENDEL, Marja JÄÄTTELÄ, Marcel LEIST, 2001. In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells. In: Biochemical and Biophysical Research Communications. Elsevier. 2001, 283(5), pp. 1111-1117. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.2001.4918
BibTex
@article{Hentze2001-05-25vitro-51266,
  year={2001},
  doi={10.1006/bbrc.2001.4918},
  title={In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells},
  number={5},
  volume={283},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={1111--1117},
  author={Hentze, Hannes and Schwoebel, Frank and Lund, Søren and Kehl, Marius and Ertel, Wolfgang and Wendel, Albrecht and Jäättelä, Marja and Leist, Marcel}
}
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