Publikation: In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
While caspases play an established role as intracellular executors of apoptosis, little is known about extracellular activities of this ubiquitously expressed family of proteases. We demonstrate here that recombinant caspase-3 retained enzymatic activity in various extracellular fluids. Experiments with cell lines, primary cells, and mice with fulminant CD95-triggered hepatitis showed that significant amounts of DEVD-aminofluoromethylcoumarine-cleaving activity, indicative of active effector caspases, were released into the medium/plasma during apoptosis. Furthermore, caspase activities were detected in liquor samples from human head trauma patients. These findings warrant closer investigation of DEVDase activity as a diagnostic marker, and of potential extracellular substrates for caspases.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
HENTZE, Hannes, Frank SCHWOEBEL, Søren LUND, Marius KEHL, Wolfgang ERTEL, Albrecht WENDEL, Marja JÄÄTTELÄ, Marcel LEIST, 2001. In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells. In: Biochemical and Biophysical Research Communications. Elsevier. 2001, 283(5), pp. 1111-1117. ISSN 0006-291X. eISSN 1090-2104. Available under: doi: 10.1006/bbrc.2001.4918BibTex
@article{Hentze2001-05-25vitro-51266, year={2001}, doi={10.1006/bbrc.2001.4918}, title={In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells}, number={5}, volume={283}, issn={0006-291X}, journal={Biochemical and Biophysical Research Communications}, pages={1111--1117}, author={Hentze, Hannes and Schwoebel, Frank and Lund, Søren and Kehl, Marius and Ertel, Wolfgang and Wendel, Albrecht and Jäättelä, Marja and Leist, Marcel} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/51266"> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-08T13:59:53Z</dc:date> <dc:creator>Jäättelä, Marja</dc:creator> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:contributor>Schwoebel, Frank</dc:contributor> <dcterms:issued>2001-05-25</dcterms:issued> <dc:contributor>Kehl, Marius</dc:contributor> <dc:contributor>Lund, Søren</dc:contributor> <dc:creator>Hentze, Hannes</dc:creator> <dc:creator>Schwoebel, Frank</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:language>eng</dc:language> <dc:creator>Ertel, Wolfgang</dc:creator> <dc:contributor>Wendel, Albrecht</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:rights>terms-of-use</dc:rights> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/51266"/> <dc:contributor>Hentze, Hannes</dc:contributor> <dc:contributor>Ertel, Wolfgang</dc:contributor> <dcterms:title>In vivo and in vitro evidence for extracellular caspase activity released from apoptotic cells</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-10-08T13:59:53Z</dcterms:available> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:abstract xml:lang="eng">While caspases play an established role as intracellular executors of apoptosis, little is known about extracellular activities of this ubiquitously expressed family of proteases. We demonstrate here that recombinant caspase-3 retained enzymatic activity in various extracellular fluids. Experiments with cell lines, primary cells, and mice with fulminant CD95-triggered hepatitis showed that significant amounts of DEVD-aminofluoromethylcoumarine-cleaving activity, indicative of active effector caspases, were released into the medium/plasma during apoptosis. Furthermore, caspase activities were detected in liquor samples from human head trauma patients. These findings warrant closer investigation of DEVDase activity as a diagnostic marker, and of potential extracellular substrates for caspases.</dcterms:abstract> <dc:creator>Leist, Marcel</dc:creator> <dc:contributor>Jäättelä, Marja</dc:contributor> <dc:creator>Kehl, Marius</dc:creator> <dc:creator>Lund, Søren</dc:creator> <dc:creator>Wendel, Albrecht</dc:creator> <dc:contributor>Leist, Marcel</dc:contributor> </rdf:Description> </rdf:RDF>