Publikation: Binding of Sodium Ions and Cardiotonic Steroids to Native and Selectively Trypsinized Na,K Pump, Detected by Charge Movements
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A fluorescent dye, RH421, has been used to characterize charge movements associated with cation and cardiotonic steroid binding to Na,K-ATPase and to a specifically trypsinized preparation, so-called "19-kDa membranes." A fluorescence decrease induced by Na+ is attributed to electrogenic binding of one Na+ ion from the cytoplasm. The apparent affinity for Na+ is the same in both preparations. (ATP + Na + Mg) or (Pi + Mg)-induced fluorescence signals observed with native enzyme are not observed in 19-kDa membranes, consistent with loss of ATP binding and phosphorylation. Cardiotonic steroids (CS) bind to native enzyme and 19-kDa membranes as judged by RH421 signals, fluorescence of anthroyl ouabain, anidn hibition of Rb+ occlusion. Binding affinities to both preparations are in the micromolar range, and bindingis prevented by the presence of Na+ or K+. The kinetics of glycone binding and dissociation are identical in both preparations, but aglycones bind and dissociate about 6-foldf aster to 19-kDa membranes. Binding of Na+ and cardiotonic steroids is inactivated upon heating or extensive Pronase digestion of 19-kDa membranes. This suggests that cation and CS binding depend on the structural integrity of a complex of the proteolytic fragments, and that sites for both cations or CS consist of ligating groups located on more than one fragments of 19-kDa membranes.
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SCHWAPPACH, Blanche, Werner STÜRMER, Hans-Jürgen APELL, Steven J. D. KARLISH, 1994. Binding of Sodium Ions and Cardiotonic Steroids to Native and Selectively Trypsinized Na,K Pump, Detected by Charge Movements. In: The Journal of Biological Chemistry. 1994, 269(26), pp. 21620-21626BibTex
@article{Schwappach1994Bindi-7774, year={1994}, title={Binding of Sodium Ions and Cardiotonic Steroids to Native and Selectively Trypsinized Na,K Pump, Detected by Charge Movements}, number={26}, volume={269}, journal={The Journal of Biological Chemistry}, pages={21620--21626}, author={Schwappach, Blanche and Stürmer, Werner and Apell, Hans-Jürgen and Karlish, Steven J. D.} }
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