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Binding of Sodium Ions and Cardiotonic Steroids to Native and Selectively Trypsinized Na,K Pump, Detected by Charge Movements

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Binding_of_Sodium_Ions.pdf
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1994

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Schwappach, Blanche
Stürmer, Werner
Karlish, Steven J. D.

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The Journal of Biological Chemistry. 1994, 269(26), pp. 21620-21626

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A fluorescent dye, RH421, has been used to characterize charge movements associated with cation and cardiotonic steroid binding to Na,K-ATPase and to a specifically trypsinized preparation, so-called "19-kDa membranes." A fluorescence decrease induced by Na+ is attributed to electrogenic binding of one Na+ ion from the cytoplasm. The apparent affinity for Na+ is the same in both preparations. (ATP + Na + Mg) or (Pi + Mg)-induced fluorescence signals observed with native enzyme are not observed in 19-kDa membranes, consistent with loss of ATP binding and phosphorylation. Cardiotonic steroids (CS) bind to native enzyme and 19-kDa membranes as judged by RH421 signals, fluorescence of anthroyl ouabain, anidn hibition of Rb+ occlusion. Binding affinities to both preparations are in the micromolar range, and bindingis prevented by the presence of Na+ or K+. The kinetics of glycone binding and dissociation are identical in both preparations, but aglycones bind and dissociate about 6-foldf aster to 19-kDa membranes. Binding of Na+ and cardiotonic steroids is inactivated upon heating or extensive Pronase digestion of 19-kDa membranes. This suggests that cation and CS binding depend on the structural integrity of a complex of the proteolytic fragments, and that sites for both cations or CS consist of ligating groups located on more than one fragments of 19-kDa membranes.

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570 Biowissenschaften, Biologie

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ISO 690SCHWAPPACH, Blanche, Werner STÜRMER, Hans-Jürgen APELL, Steven J. D. KARLISH, 1994. Binding of Sodium Ions and Cardiotonic Steroids to Native and Selectively Trypsinized Na,K Pump, Detected by Charge Movements. In: The Journal of Biological Chemistry. 1994, 269(26), pp. 21620-21626
BibTex
@article{Schwappach1994Bindi-7774,
  year={1994},
  title={Binding of Sodium Ions and Cardiotonic Steroids to Native and Selectively Trypsinized Na,K Pump, Detected by Charge Movements},
  number={26},
  volume={269},
  journal={The Journal of Biological Chemistry},
  pages={21620--21626},
  author={Schwappach, Blanche and Stürmer, Werner and Apell, Hans-Jürgen and Karlish, Steven J. D.}
}
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