Detection and Evaluation of Proliferating Cell Nuclear Antigen (PCNA) in Rat Tissue by an Improved Immunohistochemical Procedure

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1991
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Foley, Julie F.
Swenberg, James A.
Maronpot, Robert R.
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Zusammenfassung

Proliferating cell nuclear antigen (PCNA), an endogenous cell replication marker, was detected by an improved immunohistochemical procedure (super sensitive biotin-streptavidin), in formalin fixed, paraffin embedded tissue. Small intestine, liver, kidney, testis, and esophagus from rats were stained for the detection and evaluation of PCNA as asuitable marker for cell proliferation. Different patterns of staining that are believed to correlate with the individual phases of the cell cycle (G1, S, G2, M, G0) were recognized. PCNA offers an alternative to established cell labeling techniques for investigating cell proliferation.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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formalin fixation, paraffin processing, proliferating cell nuclear antigen, super sensitive biotin-streptavidin
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ISO 690FOLEY, Julie F., Daniel R. DIETRICH, James A. SWENBERG, Robert R. MARONPOT, 1991. Detection and Evaluation of Proliferating Cell Nuclear Antigen (PCNA) in Rat Tissue by an Improved Immunohistochemical Procedure. In: Journal of Histotechnology. 1991, 14(4), pp. 237-241. ISSN 0147-8885. eISSN 2046-0236. Available under: doi: 10.1179/his.1991.14.4.237
BibTex
@article{Foley1991Detec-8729,
  year={1991},
  doi={10.1179/his.1991.14.4.237},
  title={Detection and Evaluation of Proliferating Cell Nuclear Antigen (PCNA) in Rat Tissue by an Improved Immunohistochemical Procedure},
  number={4},
  volume={14},
  issn={0147-8885},
  journal={Journal of Histotechnology},
  pages={237--241},
  author={Foley, Julie F. and Dietrich, Daniel R. and Swenberg, James A. and Maronpot, Robert R.}
}
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