Aureochrome 1a is involved in the photoacclimation of the diatom Phaeodactylum tricornutum

Schellenberger Costa, Benjamin; Sachse, Matthias; Jungandreas, Anne; Río Bártulos, Carolina; Gruber, Ansgar; Jakob, Torsten; Kroth, Peter G.; Wilhelm, Christian

Aureochrome 1a is involved in the photoacclimation of the diatom Phaeodactylum tricornutum

Dateien

journal.pone.0074451 edit.pdfGröße: 5.27 MBDownloads: 1387

Datum

2013

Autor:innen

Schellenberger Costa, Benjamin

Sachse, Matthias

Jungandreas, Anne

Río Bártulos, Carolina

Gruber, Ansgar

Jakob, Torsten

Kroth, Peter G.

Wilhelm, Christian

Publikationstyp

Zeitschriftenartikel

unikn.publication.listelement.citation.prefix.version.undefined

PLoS ONE. 2013, 8(9), pp. e74451. ISSN 1932-6203. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0074451

Zusammenfassung

Aureochromes constitute a family of blue light (BL) receptors which are found exclusively in heterokont algae such as diatoms (Bacillariophyceae) and yellow-green algae (Xanthophyceae). Previous studies on the diatom Phaeodactylum tricornutum indicate that the formation of a high light acclimated phenotype is mediated by the absorption of BL and that aureochromes might play an important role in this process. P. tricornutum possesses four genes encoding aureochromes. In this study we confirm the nuclear localisation of the PtAUREO1a, 1b and 2 proteins. Furthermore we studied the physiology of light quality acclimation in genetically transformed P. tricornutum cell lines with reduced expression of the aureochrome 1a gene. The results demonstrate that the AUREO1a protein has a distinct function in light acclimation. However, rather unexpectedly AUREO1a seems to repress high light acclimation which resulted in a state of "hyper" high light acclimation in aureo1a silenced strains. This was indicated by characteristic changes of several photosynthetic parameters, including increased maximum photosynthesis rates, decreased chlorophyll a contents per cell and increased values of non-photochemical quenching in AUREO1a silenced strains compared to wild type cultures. Strikingly, AUREO1a silenced strains exhibited phenotypic differences compared to wild type cells during cultivation under BL as well as under red light (RL) conditions. Therefore, AUREO1a might influence the RL signalling process, suggesting an interaction of AUREO1a with RL perception pathways.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

SCHELLENBERGER COSTA, Benjamin, Matthias SACHSE, Anne JUNGANDREAS, Carolina RÍO BÁRTULOS, Ansgar GRUBER, Torsten JAKOB, Peter G. KROTH, Christian WILHELM, 2013. Aureochrome 1a is involved in the photoacclimation of the diatom Phaeodactylum tricornutum. In: PLoS ONE. 2013, 8(9), pp. e74451. ISSN 1932-6203. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0074451

BibTex

@article{SchellenbergerCosta2013Aureo-25826,
  year={2013},
  doi={10.1371/journal.pone.0074451},
  title={Aureochrome 1a is involved in the photoacclimation of the diatom Phaeodactylum tricornutum},
  number={9},
  volume={8},
  issn={1932-6203},
  journal={PLoS ONE},
  author={Schellenberger Costa, Benjamin and Sachse, Matthias and Jungandreas, Anne and Río Bártulos, Carolina and Gruber, Ansgar and Jakob, Torsten and Kroth, Peter G. and Wilhelm, Christian},
  note={Article Number: e74451}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/25826">
    <dc:contributor>Río Bártulos, Carolina</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Río Bártulos, Carolina</dc:creator>
    <dc:contributor>Jungandreas, Anne</dc:contributor>
    <dc:creator>Jungandreas, Anne</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-01-13T13:24:14Z</dc:date>
    <dc:contributor>Sachse, Matthias</dc:contributor>
    <dc:creator>Jakob, Torsten</dc:creator>
    <dc:contributor>Schellenberger Costa, Benjamin</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/25826/1/journal.pone.0074451%20edit.pdf"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/25826"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/25826/1/journal.pone.0074451%20edit.pdf"/>
    <dc:contributor>Gruber, Ansgar</dc:contributor>
    <dcterms:abstract xml:lang="eng">Aureochromes constitute a family of blue light (BL) receptors which are found exclusively in heterokont algae such as diatoms (Bacillariophyceae) and yellow-green algae (Xanthophyceae). Previous studies on the diatom Phaeodactylum tricornutum indicate that the formation of a high light acclimated phenotype is mediated by the absorption of BL and that aureochromes might play an important role in this process. P. tricornutum possesses four genes encoding aureochromes. In this study we confirm the nuclear localisation of the PtAUREO1a, 1b and 2 proteins. Furthermore we studied the physiology of light quality acclimation in genetically transformed P. tricornutum cell lines with reduced expression of the aureochrome 1a gene. The results demonstrate that the AUREO1a protein has a distinct function in light acclimation. However, rather unexpectedly AUREO1a seems to repress high light acclimation which resulted in a state of "hyper" high light acclimation in aureo1a silenced strains. This was indicated by characteristic changes of several photosynthetic parameters, including increased maximum photosynthesis rates, decreased chlorophyll a contents per cell and increased values of non-photochemical quenching in AUREO1a silenced strains compared to wild type cultures. Strikingly, AUREO1a silenced strains exhibited phenotypic differences compared to wild type cells during cultivation under BL as well as under red light (RL) conditions. Therefore, AUREO1a might influence the RL signalling process, suggesting an interaction of AUREO1a with RL perception pathways.</dcterms:abstract>
    <dc:creator>Schellenberger Costa, Benjamin</dc:creator>
    <dc:contributor>Kroth, Peter G.</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2014-01-13T13:24:14Z</dcterms:available>
    <dc:creator>Wilhelm, Christian</dc:creator>
    <dcterms:issued>2013</dcterms:issued>
    <dcterms:title>Aureochrome 1a is involved in the photoacclimation of the diatom Phaeodactylum tricornutum</dcterms:title>
    <dc:contributor>Wilhelm, Christian</dc:contributor>
    <dc:creator>Kroth, Peter G.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Sachse, Matthias</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Gruber, Ansgar</dc:creator>
    <dcterms:bibliographicCitation>PLoS ONE ; 8 (2013), 9. - e74451</dcterms:bibliographicCitation>
    <dc:contributor>Jakob, Torsten</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja