Publikation: Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1
Dateien
Datum
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
When Streptomyces violaceoruber grows together with Streptomyces sp. MG7-G1, it reacts with strongly induced droplet production on its aerial mycelium. Initially the metabolite profile of droplets from S. violaceoruber in co-culture with Streptomyces sp. MG7-G1 was compared to samples from S. violaceoruber in single-culture using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Then, also the exudate from agar plates of co-cultures and single cultures (after freezing and thawing) was analysed. Several compounds were only observed when S. violaceoruber was grown in co-culture. Based on their high-resolution ESI mass spectra and their comparable retention times to the calcium-dependent antibiotics (CDAs) produced by S. violaceo-ruber the new compounds were suspected to be deacylated calcium-dependent antibiotics (daCDAs), lacking the 2,3-epoxyhexanoyl residue of CDAs. This was verified by detailed analysis of the MS/MS spectra of the deacylated CDAs in comparison to the CDAs. The major CDA compounds present in calcium ion supplemented agar medium of co-cultures were daCDAs, suggesting that Streptomyces sp. MG7-G1 expresses a deacylase that degrades CDAs.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
SCHINDL, Kathrin, Deepika SHARMA, Dieter SPITELLER, 2020. Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1. In: ChemBioChem. Wiley. 2020, 21(21), pp. 3151-3157. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.202000404BibTex
@article{Schindl2020-11-02Deacy-50082,
year={2020},
doi={10.1002/cbic.202000404},
title={Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1},
number={21},
volume={21},
issn={1439-4227},
journal={ChemBioChem},
pages={3151--3157},
author={Schindl, Kathrin and Sharma, Deepika and Spiteller, Dieter}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/50082">
<dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dc:contributor>Spiteller, Dieter</dc:contributor>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/50082/1/Schindl_2-1v4iz900qs99f8.pdf"/>
<dcterms:title>Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1</dcterms:title>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/50082/1/Schindl_2-1v4iz900qs99f8.pdf"/>
<dcterms:issued>2020-11-02</dcterms:issued>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-07-01T11:03:13Z</dc:date>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dc:creator>Spiteller, Dieter</dc:creator>
<dc:creator>Sharma, Deepika</dc:creator>
<dc:creator>Schindl, Kathrin</dc:creator>
<dc:contributor>Schindl, Kathrin</dc:contributor>
<dc:contributor>Sharma, Deepika</dc:contributor>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-07-01T11:03:13Z</dcterms:available>
<dcterms:abstract xml:lang="eng">When Streptomyces violaceoruber grows together with Streptomyces sp. MG7-G1, it reacts with strongly induced droplet production on its aerial mycelium. Initially the metabolite profile of droplets from S. violaceoruber in co-culture with Streptomyces sp. MG7-G1 was compared to samples from S. violaceoruber in single-culture using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Then, also the exudate from agar plates of co-cultures and single cultures (after freezing and thawing) was analysed. Several compounds were only observed when S. violaceoruber was grown in co-culture. Based on their high-resolution ESI mass spectra and their comparable retention times to the calcium-dependent antibiotics (CDAs) produced by S. violaceo-ruber the new compounds were suspected to be deacylated calcium-dependent antibiotics (daCDAs), lacking the 2,3-epoxyhexanoyl residue of CDAs. This was verified by detailed analysis of the MS/MS spectra of the deacylated CDAs in comparison to the CDAs. The major CDA compounds present in calcium ion supplemented agar medium of co-cultures were daCDAs, suggesting that Streptomyces sp. MG7-G1 expresses a deacylase that degrades CDAs.</dcterms:abstract>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:language>eng</dc:language>
<bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/50082"/>
<dc:rights>Attribution 4.0 International</dc:rights>
</rdf:Description>
</rdf:RDF>
