Publikation: Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1
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When Streptomyces violaceoruber grows together with Streptomyces sp. MG7-G1, it reacts with strongly induced droplet production on its aerial mycelium. Initially the metabolite profile of droplets from S. violaceoruber in co-culture with Streptomyces sp. MG7-G1 was compared to samples from S. violaceoruber in single-culture using high-performance liquid chromatography-mass spectrometry (HPLC-MS). Then, also the exudate from agar plates of co-cultures and single cultures (after freezing and thawing) was analysed. Several compounds were only observed when S. violaceoruber was grown in co-culture. Based on their high-resolution ESI mass spectra and their comparable retention times to the calcium-dependent antibiotics (CDAs) produced by S. violaceo-ruber the new compounds were suspected to be deacylated calcium-dependent antibiotics (daCDAs), lacking the 2,3-epoxyhexanoyl residue of CDAs. This was verified by detailed analysis of the MS/MS spectra of the deacylated CDAs in comparison to the CDAs. The major CDA compounds present in calcium ion supplemented agar medium of co-cultures were daCDAs, suggesting that Streptomyces sp. MG7-G1 expresses a deacylase that degrades CDAs.
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SCHINDL, Kathrin, Deepika SHARMA, Dieter SPITELLER, 2020. Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1. In: ChemBioChem. Wiley. 2020, 21(21), pp. 3151-3157. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.202000404BibTex
@article{Schindl2020-11-02Deacy-50082, year={2020}, doi={10.1002/cbic.202000404}, title={Deacylation of calcium-dependent antibiotics from Streptomyces violaceoruber in co-culture with Streptomyces sp. MG7-G1}, number={21}, volume={21}, issn={1439-4227}, journal={ChemBioChem}, pages={3151--3157}, author={Schindl, Kathrin and Sharma, Deepika and Spiteller, Dieter} }
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