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Differential Regulation of a Hyperthermophilic α-Amylase with a Novel (Ca,Zn) Two-metal Center by Zinc

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2003

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Linden, Anni
Meyer-Klaucke, Wolfram
Antranikian, Garabed
Wilmanns, Matthias

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The Journal of Biological Chemistry : JBC. 2003, 278(11), pp. 9875-9884. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M211339200

Zusammenfassung

The crystal structure of the alpha-amylase from the hyperthermophilic archaeon Pyrococcus woesei was solved in the presence of three inhibitors: acarbose, Tris, and zinc. In the absence of exogenous metals, this alpha-amylase bound 1 and 4 molar eq of zinc and calcium, respectively. The structure reveals a novel, activating, two-metal (Ca,Zn)-binding site and a second inhibitory zinc-binding site that is found in the -1 sugar-binding pocket within the active site. The data resolve the apparent paradox between the zinc requirement for catalytic activity and its strong inhibitory effect when added in molar excess. They provide a rationale as to why this alpha-amylase, in contrast to commercially available alpha-amylases, does not require the addition of metal ions for full catalytic activity, suggesting it as an ideal target to maximize the efficiency of industrial processes like liquefaction of starch.

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570 Biowissenschaften, Biologie

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ISO 690LINDEN, Anni, Olga MAYANS, Wolfram MEYER-KLAUCKE, Garabed ANTRANIKIAN, Matthias WILMANNS, 2003. Differential Regulation of a Hyperthermophilic α-Amylase with a Novel (Ca,Zn) Two-metal Center by Zinc. In: The Journal of Biological Chemistry : JBC. 2003, 278(11), pp. 9875-9884. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M211339200
BibTex
@article{Linden2003-03-14Diffe-42039,
  year={2003},
  doi={10.1074/jbc.M211339200},
  title={Differential Regulation of a Hyperthermophilic α-Amylase with a Novel (Ca,Zn) Two-metal Center by Zinc},
  number={11},
  volume={278},
  issn={0021-9258},
  journal={The Journal of Biological Chemistry : JBC},
  pages={9875--9884},
  author={Linden, Anni and Mayans, Olga and Meyer-Klaucke, Wolfram and Antranikian, Garabed and Wilmanns, Matthias}
}
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