Artificial Ribozyme-Based Regulators of Gene Expression

Kurzanzeige
dc.contributor.authorHolder, Isabelle T.
dc.contributor.authorHartig, Jörg S.
dc.date.accessioned2011-09-22T09:39:32Zdeu
dc.date.available2011-09-22T09:39:32Zdeu
dc.date.issued2011
dc.description.abstractThe development of RNA-based switches of gene expression is summarized. Most switches are based on the Schistosoma mansonii hammerhead ribozyme (HHR), a self-cleaving RNA sequence that is inserted into an mRNA. Control of HHR self-cleavage is achieved by the attachment of an aptamer domain to the HHR scaffold. External addition of a small-molecule ligand regulates catalytic activity of the ribozyme and hence gene expression. These so-called aptazymes are suited to control several classes of RNA. In addition to mRNA, we have incorporated artificial RNA switches into tRNA as well as the bacterial 16S rRNA. In addition, the presented switches should be widely applicable as demonstrated by experiments in bacteria, yeast, and mammals.eng
dc.description.versionpublished
dc.identifier.citationSynlett (2011), 11. - S. 1486-1494deu
dc.identifier.doi10.1055/s-0030-1260583deu
dc.identifier.ppn377517623deu
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dc.language.isoengdeu
dc.legacy.dateIssued2011-09-22deu
dc.rightsterms-of-usedeu
dc.rights.urihttps://rightsstatements.org/page/InC/1.0/deu
dc.subject.ddc540deu
dc.titleArtificial Ribozyme-Based Regulators of Gene Expressioneng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
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  volume={2011},
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  journal={Synlett},
  pages={1486--1494},
  author={Holder, Isabelle T. and Hartig, Jörg S.}
}
kops.citation.iso690HOLDER, Isabelle T., Jörg S. HARTIG, 2011. Artificial Ribozyme-Based Regulators of Gene Expression. In: Synlett. 2011, 2011(11), pp. 1486-1494. ISSN 0936-5214. eISSN 1437-2096. Available under: doi: 10.1055/s-0030-1260583deu
kops.citation.iso690HOLDER, Isabelle T., Jörg S. HARTIG, 2011. Artificial Ribozyme-Based Regulators of Gene Expression. In: Synlett. 2011, 2011(11), pp. 1486-1494. ISSN 0936-5214. eISSN 1437-2096. Available under: doi: 10.1055/s-0030-1260583eng
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kops.submitter.emailastrid.joachimi@uni-konstanz.dedeu
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