Publikation: Performance of a fast fiber based UV/Vis multiwavelength detector for the analytical ultracentrifuge
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Datum
2008
Autor:innen
Strauss, Holger M.
Karabudak, Engin
Bhattacharyya, Saroj
Kretzschmar, Andreas
Wohlleben, Wendel
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Colloid and Polymer Science. 2008, 286(2), pp. 121-128. ISSN 0303-402X. eISSN 1435-1536. Available under: doi: 10.1007/s00396-007-1815-5
Zusammenfassung
The optical setup and the performance of a prototype UV/Vis multiwavelength analytical ultracentrifuge (MWL-AUC) is described and compared to the commercially available Optima XL-A from Beckman Coulter. Slight modifications have been made to the optical path of the MWL-AUC. With respect to wavelength accuracy and radial resolution, the new MWL-AUC is found to be comparable to the existing XL-A. Absorbance accuracy is dependent on the light intensity available at the detection wavelength as well as the intrinsic noise of the data. Measurements from single flashes of light are more noisy for the MWL-AUC, potentially due to the absence of flash-to-flash normalization in the current design. However, the possibility of both wavelength and scan averaging can compensate for this and still give much faster scan rates than the XL-A. Some further improvements of the existing design are suggested based on these findings.
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STRAUSS, Holger M., Engin KARABUDAK, Saroj BHATTACHARYYA, Andreas KRETZSCHMAR, Wendel WOHLLEBEN, Helmut CÖLFEN, 2008. Performance of a fast fiber based UV/Vis multiwavelength detector for the analytical ultracentrifuge. In: Colloid and Polymer Science. 2008, 286(2), pp. 121-128. ISSN 0303-402X. eISSN 1435-1536. Available under: doi: 10.1007/s00396-007-1815-5BibTex
@article{Strauss2008Perfo-40368,
year={2008},
doi={10.1007/s00396-007-1815-5},
title={Performance of a fast fiber based UV/Vis multiwavelength detector for the analytical ultracentrifuge},
number={2},
volume={286},
issn={0303-402X},
journal={Colloid and Polymer Science},
pages={121--128},
author={Strauss, Holger M. and Karabudak, Engin and Bhattacharyya, Saroj and Kretzschmar, Andreas and Wohlleben, Wendel and Cölfen, Helmut}
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<dcterms:abstract xml:lang="eng">The optical setup and the performance of a prototype UV/Vis multiwavelength analytical ultracentrifuge (MWL-AUC) is described and compared to the commercially available Optima XL-A from Beckman Coulter. Slight modifications have been made to the optical path of the MWL-AUC. With respect to wavelength accuracy and radial resolution, the new MWL-AUC is found to be comparable to the existing XL-A. Absorbance accuracy is dependent on the light intensity available at the detection wavelength as well as the intrinsic noise of the data. Measurements from single flashes of light are more noisy for the MWL-AUC, potentially due to the absence of flash-to-flash normalization in the current design. However, the possibility of both wavelength and scan averaging can compensate for this and still give much faster scan rates than the XL-A. Some further improvements of the existing design are suggested based on these findings.</dcterms:abstract>
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