Comparison of nitrogen nutrition and soil carbon status of afforested stands established in degraded soil of the Loess Plateau, China

Abstract

The Loess Plateau in China constitutes an area short of soil nitrogen and organic carbon due to local land degradation induced by various factors (i.e. long term changes of land use, climate conditions, and soil properties). The present study aimed to examine the effects of species and land management by afforestation on tree N acquisition capacity and soil N and C availability in degraded soils of the Loess Plateau area. We quantified root N uptake of inorganic and organic N sources as well as soil N and C availability, both at the tree species (i.e. Robinia pseudoacacia L., Juglans regia L. and Pinus tabulaeformis Carr.) and the land management (i.e. arable vs. monoculture vs. mixed afforested stands) levels. Our results indicated that afforestation improved soil N and organic C availabilities compared to abandoned arable land (p < 0.05). In particular, the presence of N2-fixing R. pseudoacacia enhanced root N concentrations (ca. 3.0 times) and soil NO3- (ca. 5.4 times), soil total N (ca. 1.9 times) and organic C (ca. 3.4 times) availabilities, but decreased soil NH4+ (ca. −33%), microbial biomass carbon (ca. −74%) and nitrogen (ca. −54%) in the mixed stand compared to J. regia monoculture. Under the experimental conditions applied, the afforested trees preferred organic over inorganic N compounds as well as NO3− over NH4+; J. regia in monoculture had a highest root amino acids N uptake capacities (i.e. 76.6 ± 7.7 nmol N (g fw)−1 h−1 for glutamine, 90.3 ± 8.9 nmol N (g fw)−1 h−1 for arginine) compared to other tree species whereas such high uptake capacities were largely repressed in the mixed stand with R. pseudoacacia. Thus, in the Loess Plateau area, the inter-planting system of J. regia with N2-fixing R. pseudoacacia could improve the total soil N and organic C pools as well as plant N cycling compared to traditional arable land use and J. regia monoculture system. This study shows that inter-planting R. pseudoacacia with economic fruit trees can be considered a successful strategy for soil regeneration by afforestation in future land management projects.