(Na+K)-ATPase in artificial lipid vesicles : influence of the concentration of mono- and divalent cations on the pumping rate

Kurzanzeige
dc.contributor.authorApell, Hans-Jürgen
dc.contributor.authorMarcus, Madeleine M.deu
dc.date.accessioned2011-03-24T17:40:45Zdeu
dc.date.available2011-03-24T17:40:45Zdeu
dc.date.issued1986deu
dc.description.abstract(Na,K)-ATPase from kidney outer medulla was incorporated into artificial dioleoylphosphatidylcholine vesicles. Transport activity was induced by adding ATP to the external medium. A voltage-sensitive dye was used to detect the ATP-driven potassium extrusion in the presence of valinomycin. The observed substrate-protein interactions of the reconstituted (Na+K)-ATPase largely agree with that from native tissues. An agreement between ATP hydrolysis and transport activity is given for concentration dependences of sodium, potassium, magnesium and calcium ions. The only significant deviations were observed in the influence of pH. Protons were found to have different influence on transport, enzymatic activity and phosphorylation of the enzyme. The transport studies showed a twofold interaction of protons with the protein: (1) competition with sodium at the cytoplasmic ion binding sites, (2) a non competitive inhibition of transport which is not correlated with protein phosphorylation.eng
dc.description.versionpublished
dc.format.mimetypeapplication/pdfdeu
dc.identifier.citationFirst publ. in: Biochimica et Biophysica Acta - Biomembranes 862 (1986), pp. 254-264deu
dc.identifier.doi10.1016/0005-2736(86)90226-9
dc.identifier.ppn273348507deu
dc.identifier.urihttp://kops.uni-konstanz.de/handle/123456789/8119
dc.language.isoengdeu
dc.legacy.dateIssued2007deu
dc.rightsAttribution-NonCommercial-NoDerivs 2.0 Generic
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/
dc.subject(Na,K)-ATPasedeu
dc.subjectMembrane reconstitutiondeu
dc.subjectKineticsdeu
dc.subjectCation concentration dependencedeu
dc.subjectpH effectdeu
dc.subjectPumping activitydeu
dc.subject(Rabbit kidney)deu
dc.subject.ddc570deu
dc.title(Na+K)-ATPase in artificial lipid vesicles : influence of the concentration of mono- and divalent cations on the pumping rateeng
dc.typeJOURNAL_ARTICLEdeu
dspace.entity.typePublication
kops.citation.bibtex
@article{Apell1986NaKAT-8119,
  year={1986},
  doi={10.1016/0005-2736(86)90226-9},
  title={(Na+K)-ATPase in artificial lipid vesicles : influence of the concentration of mono- and divalent cations on the pumping rate},
  number={2},
  volume={862},
  issn={0005-2736},
  journal={Biochimica et Biophysica Acta - Biomembranes},
  pages={254--264},
  author={Apell, Hans-Jürgen and Marcus, Madeleine M.}
}
kops.citation.iso690APELL, Hans-Jürgen, Madeleine M. MARCUS, 1986. (Na+K)-ATPase in artificial lipid vesicles : influence of the concentration of mono- and divalent cations on the pumping rate. In: Biochimica et Biophysica Acta - Biomembranes. 1986, 862(2), pp. 254-264. ISSN 0005-2736. Available under: doi: 10.1016/0005-2736(86)90226-9deu
kops.citation.iso690APELL, Hans-Jürgen, Madeleine M. MARCUS, 1986. (Na+K)-ATPase in artificial lipid vesicles : influence of the concentration of mono- and divalent cations on the pumping rate. In: Biochimica et Biophysica Acta - Biomembranes. 1986, 862(2), pp. 254-264. ISSN 0005-2736. Available under: doi: 10.1016/0005-2736(86)90226-9eng
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