Publikation: The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition
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PA28α/β is a regulatory complex of the 20S proteasome which consists of two IFN-γ inducible subunits. Both subunits, α and β, contribute equally to the formation of hexa- or heptameric rings which can associate with the 20S proteasome. Previously, we have shown that overexpression of the PA28α subunit enhanced the MHC class I-restricted presentation of two viral epitopes and that purified PA28α/β accelerated T cell epitope generation by the 20S proteasome in vitro, indicating a role for PA28α/β in antigen presentation. This conclusion was recently confirmed in PA28β gene targeted mice which were severely deficient in MHC class I-restricted antigen presentation. These mice displayed a defect in the assembly of immunoproteasomes, suggesting that a lack of the proteasome subunits LMP2, LMP7, and MECL-1 may account for the deficiency in antigen presentation. In this study we investigated whether the effect of PA28α/β on antigen presentation is dependent on a change of proteasome subunit composition. We have analyzed the assembly and subunit composition of proteasomes in fibroblast transfectants overexpressing both, α and β subunits of PA28. In these transfectants we found a marked enhancement in the presentation of the immunodominant H-2Ld-restricted pp89 epitope of murine cytomegalovirus, although the 20S proteasome composition was the same as in recipient cells. We, therefore, conclude that PA28α/β can enhance antigen processing independently of changes in 20S proteasome subunit composition or assembly.
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SCHWARZ, Katrin, Maren EGGERS, Andrea SOZA, Ulrich H. KOSZINOWSKI, Peter-M. KLÖTZEL, Marcus GRÖTTRUP, 2000. The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition. In: European Journal of Immunology. 2000, 30(12), pp. 3672-3679. ISSN 0014-2980. eISSN 1521-4141. Available under: doi: 10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>3.0.CO;2-BBibTex
@article{Schwarz2000prote-22161, year={2000}, doi={10.1002/1521-4141(200012)30:12<3672::AID-IMMU3672>3.0.CO;2-B}, title={The proteasome regulator PA28α/β can enhance antigen presentation without affecting 20S proteasome subunit composition}, number={12}, volume={30}, issn={0014-2980}, journal={European Journal of Immunology}, pages={3672--3679}, author={Schwarz, Katrin and Eggers, Maren and Soza, Andrea and Koszinowski, Ulrich H. and Klötzel, Peter-M. and Gröttrup, Marcus} }
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