Interactome of Site-Specifically Acetylated Linker Histone H1

Kurzanzeige
dc.contributor.authorHöllmüller, Eva
dc.contributor.authorGreiner, Katharina
dc.contributor.authorKienle, Simon Maria
dc.contributor.authorScheffner, Martin
dc.contributor.authorMarx, Andreas
dc.contributor.authorStengel, Florian
dc.date.accessioned2021-09-23T11:17:25Z
dc.date.available2021-09-23T11:17:25Z
dc.date.issued2021-09-03eng
dc.description.abstractLinker histone H1 plays a key role in chromatin organization and maintenance, yet our knowledge of the regulation of H1 functions by post-translational modifications is rather limited. In this study, we report on the generation of site-specifically mono- and di-acetylated linker histone H1.2 by genetic code expansion. We used these modified histones to identify and characterize the acetylation-dependent cellular interactome of H1.2 by affinity purification mass spectrometry and show that site-specific acetylation results in overlapping but distinct groups of interacting partners. Among these, we find multiple translational initiation factors and transcriptional regulators such as the NAD+-dependent deacetylase SIRT1, which we demonstrate to act on acetylated H1.2. Taken together, our data suggest that site-specific acetylation of H1.2 plays a role in modulating protein–protein interactions.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1021/acs.jproteome.1c00396eng
dc.identifier.pmid34351766eng
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/54975
dc.language.isoengeng
dc.subject.ddc570eng
dc.titleInteractome of Site-Specifically Acetylated Linker Histone H1eng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Hollmuller2021-09-03Inter-54975,
  title={Interactome of Site-Specifically Acetylated Linker Histone H1},
  year={2021},
  doi={10.1021/acs.jproteome.1c00396},
  number={9},
  volume={20},
  issn={1535-3893},
  journal={Journal of Proteome Research},
  pages={4443--4451},
  author={Höllmüller, Eva and Greiner, Katharina and Kienle, Simon Maria and Scheffner, Martin and Marx, Andreas and Stengel, Florian}
}
kops.citation.iso690HÖLLMÜLLER, Eva, Katharina GREINER, Simon Maria KIENLE, Martin SCHEFFNER, Andreas MARX, Florian STENGEL, 2021. Interactome of Site-Specifically Acetylated Linker Histone H1. In: Journal of Proteome Research. American Chemical Society (ACS). 2021, 20(9), S. 4443-4451. ISSN 1535-3893. eISSN 1535-3907. Verfügbar unter: doi: 10.1021/acs.jproteome.1c00396deu
kops.citation.iso690HÖLLMÜLLER, Eva, Katharina GREINER, Simon Maria KIENLE, Martin SCHEFFNER, Andreas MARX, Florian STENGEL, 2021. Interactome of Site-Specifically Acetylated Linker Histone H1. In: Journal of Proteome Research. American Chemical Society (ACS). 2021, 20(9), pp. 4443-4451. ISSN 1535-3893. eISSN 1535-3907. Available under: doi: 10.1021/acs.jproteome.1c00396eng
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