Publikation: Crosslinking and Mass Spectrometry : An Integrated Technology to Understand the Structure and Function of Molecular Machines
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2016
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Trends in Biochemical Sciences. 2016, 41(1), pp. 20-32. ISSN 0968-0004. eISSN 1362-4326. Available under: doi: 10.1016/j.tibs.2015.10.008
Zusammenfassung
In recent years, chemical crosslinking of protein complexes and the identification of crosslinked residues by mass spectrometry (XL-MS; sometimes abbreviated as CX-MS) has become an important technique bridging mass spectrometry (MS) and structural biology. By now, XL-MS is well established and supported by publicly available resources as a convenient and versatile part of the structural biologist's toolbox. The combination of XL-MS with cryo-electron microscopy (cryo-EM) and/or integrative modeling is particularly promising to study the topology and structure of large protein assemblies. Among the targets studied so far are proteasomes, ribosomes, polymerases, chromatin remodelers, and photosystem complexes. Here we provide an overview of recent advances in XL-MS, the current state of the field, and a cursory outlook on future challenges.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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chemical crosslinking, mass spectrometry, hybrid structural biology, protein complexes
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LEITNER, Alexander, Marco FAINI, Florian STENGEL, Ruedi AEBERSOLD, 2016. Crosslinking and Mass Spectrometry : An Integrated Technology to Understand the Structure and Function of Molecular Machines. In: Trends in Biochemical Sciences. 2016, 41(1), pp. 20-32. ISSN 0968-0004. eISSN 1362-4326. Available under: doi: 10.1016/j.tibs.2015.10.008BibTex
@article{Leitner2016Cross-33538,
year={2016},
doi={10.1016/j.tibs.2015.10.008},
title={Crosslinking and Mass Spectrometry : An Integrated Technology to Understand the Structure and Function of Molecular Machines},
number={1},
volume={41},
issn={0968-0004},
journal={Trends in Biochemical Sciences},
pages={20--32},
author={Leitner, Alexander and Faini, Marco and Stengel, Florian and Aebersold, Ruedi}
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