Publikation:

A Simple and Effective Transplantation Device for Zebrafish Embryos

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2021

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Journal of Visualized Experiments (JoVE). JoVE. 2021, 174(174), e62767. eISSN 1940-087X. Available under: doi: 10.3791/62767

Zusammenfassung

Classical embryological manipulations, such as removing cells and transplanting cells within or between embryos, are powerful techniques to study complex developmental processes. Zebrafish embryos are ideally suited for these manipulations since they are easily accessible, relatively large in size, and transparent. However, previously developed devices for cell removal and transplantation are cumbersome to use or expensive to purchase. In contrast, the transplantation device presented here is economical, easy to assemble, and simple to use. In this protocol, we first introduce the handling of the transplantation device as well as its assembly from commercially and widely available parts. We then present three applications for its use: generation of ectopic clones to study signal dispersal from localized sources, extirpation of cells to produce size-reduced embryos, and germline transplantation to generate maternal-zygotic mutants. Finally, we show that the tool can also be used for embryological manipulations in other species such as the Japanese rice fish medaka.

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570 Biowissenschaften, Biologie

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ISO 690SOH, Gary H., Anna C. KÖGLER, Patrick MÜLLER, 2021. A Simple and Effective Transplantation Device for Zebrafish Embryos. In: Journal of Visualized Experiments (JoVE). JoVE. 2021, 174(174), e62767. eISSN 1940-087X. Available under: doi: 10.3791/62767
BibTex
@article{Soh2021-08-02Simpl-54685,
  year={2021},
  doi={10.3791/62767},
  title={A Simple and Effective Transplantation Device for Zebrafish Embryos},
  number={174},
  volume={174},
  journal={Journal of Visualized Experiments (JoVE)},
  author={Soh, Gary H. and Kögler, Anna C. and Müller, Patrick},
  note={Article Number: e62767}
}
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