Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid

Kugele, Anandi; Braun, Theresa S.; Widder, Pia; Williams, Lara; Schmidt, Moritz J.; Summerer, Daniel; Drescher, Malte

Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid

Dateien

Kugele_2-1cwdso3j01b388.pdfGröße: 1.23 MBDownloads: 261

Datum

2019

Angaben zur Forschungsförderung

European Union (EU): 772027

Projekt

SPICE: Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Chemical Communications. 2019, 55(13), pp. 1923-1926. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C8CC09325C

Zusammenfassung

We report site-directed protein spin labelling via Suzuki–Miyaura coupling of a nitroxide boronic acid label with the genetically encoded amino acid 4-iodo-L-phenylalanine. The resulting spin label bears a rigid biphenyl linkage with lower flexibility than spin label R1. It is suitable to obtain defined electron paramagnetic resonance distance distributions and to report protein–membrane interactions and conformational transitions of α-synuclein.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

KUGELE, Anandi, Theresa S. BRAUN, Pia WIDDER, Lara WILLIAMS, Moritz J. SCHMIDT, Daniel SUMMERER, Malte DRESCHER, 2019. Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid. In: Chemical Communications. 2019, 55(13), pp. 1923-1926. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C8CC09325C

BibTex

@article{Kugele2019-02-07Sited-44870,
  year={2019},
  doi={10.1039/C8CC09325C},
  title={Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid},
  number={13},
  volume={55},
  issn={1359-7345},
  journal={Chemical Communications},
  pages={1923--1926},
  author={Kugele, Anandi and Braun, Theresa S. and Widder, Pia and Williams, Lara and Schmidt, Moritz J. and Summerer, Daniel and Drescher, Malte}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/44870">
    <dc:language>eng</dc:language>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Drescher, Malte</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Widder, Pia</dc:contributor>
    <dc:contributor>Drescher, Malte</dc:contributor>
    <dc:creator>Summerer, Daniel</dc:creator>
    <dc:creator>Braun, Theresa S.</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:abstract xml:lang="eng">We report site-directed protein spin labelling via Suzuki–Miyaura coupling of a nitroxide boronic acid label with the genetically encoded amino acid 4-iodo-L-phenylalanine. The resulting spin label bears a rigid biphenyl linkage with lower flexibility than spin label R1. It is suitable to obtain defined electron paramagnetic resonance distance distributions and to report protein–membrane interactions and conformational transitions of α-synuclein.</dcterms:abstract>
    <dc:contributor>Kugele, Anandi</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Braun, Theresa S.</dc:contributor>
    <dcterms:issued>2019-02-07</dcterms:issued>
    <dc:contributor>Summerer, Daniel</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-02-06T09:01:54Z</dcterms:available>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/44870/1/Kugele_2-1cwdso3j01b388.pdf"/>
    <dc:rights>terms-of-use</dc:rights>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/44870"/>
    <dc:creator>Kugele, Anandi</dc:creator>
    <dc:contributor>Williams, Lara</dc:contributor>
    <dc:creator>Schmidt, Moritz J.</dc:creator>
    <dc:contributor>Schmidt, Moritz J.</dc:contributor>
    <dc:creator>Williams, Lara</dc:creator>
    <dcterms:title>Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid</dcterms:title>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-02-06T09:01:54Z</dc:date>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/44870/1/Kugele_2-1cwdso3j01b388.pdf"/>
    <dc:creator>Widder, Pia</dc:creator>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja

Begutachtet

Ja