Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid

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SPICE: Spectroscopy in cells with tailored in-vivo labelling strategies and multiply addressable nano-structural probes
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Chemical Communications. 2019, 55(13), pp. 1923-1926. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C8CC09325C
Zusammenfassung

We report site-directed protein spin labelling via Suzuki–Miyaura coupling of a nitroxide boronic acid label with the genetically encoded amino acid 4-iodo-L-phenylalanine. The resulting spin label bears a rigid biphenyl linkage with lower flexibility than spin label R1. It is suitable to obtain defined electron paramagnetic resonance distance distributions and to report protein–membrane interactions and conformational transitions of α-synuclein.

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ISO 690KUGELE, Anandi, Theresa S. BRAUN, Pia WIDDER, Lara WILLIAMS, Moritz J. SCHMIDT, Daniel SUMMERER, Malte DRESCHER, 2019. Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid. In: Chemical Communications. 2019, 55(13), pp. 1923-1926. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C8CC09325C
BibTex
@article{Kugele2019-02-07Sited-44870,
  year={2019},
  doi={10.1039/C8CC09325C},
  title={Site-directed spin labelling of proteins by Suzuki–Miyaura coupling via a genetically encoded aryliodide amino acid},
  number={13},
  volume={55},
  issn={1359-7345},
  journal={Chemical Communications},
  pages={1923--1926},
  author={Kugele, Anandi and Braun, Theresa S. and Widder, Pia and Williams, Lara and Schmidt, Moritz J. and Summerer, Daniel and Drescher, Malte}
}
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